DNA倍体分析与细胞学检测对良恶性浆膜腔积液诊断的比较  

Comparison between DNA ploidy analysis and cytology in the diagnosis of benign and malignant serous cavity effusion

作　　者：何秋阳 钟国梁 杨国顺 张灿清 马顺高 HE Qiuyang;ZHONG Guoliang;YANG Guoshun;ZHANG Canqiu;MA Shungao(Clinical Laboratory Department,PeopleDs Hospital of Dali Bai Autonomous Prefecture,DaLi,YunNan,671000,China.)

机构地区：[1]大理白族自治州人民医院检验科,云南大理671000

出　　处：《实验与检验医学》2018年第3期317-319,共3页Experimental and Laboratory Medicine

摘　　要：目的探讨DNA倍体分析技术在良恶性浆膜腔积液诊断中的临床价值。方法收集经临床确诊的95例良性及68例恶性浆膜腔积液样本,分别应用流式细胞术及显微镜对每份样本中的脱落细胞进行DNA倍体分析及形态学检查。结果DNA倍体分析及细胞学检查对恶性浆膜腔积液诊断的灵敏度分别为75%和42.6%,阴性预测值分别为84.5%和70.9%,具有显著差异(P<0.01);DNA倍体分析及细胞学检查对恶性浆膜腔积液诊断的特异性分别为97.9%和100%,阳性预测值为96.2%和阳性预测值为100%,差异无统计学意义。这两种方法的联合灵敏度为95.6%,显著高于单一检测方法 (P<0.05),联合特异性为100%,与单一检测方法相比无差异。结论基于流式细胞术的DNA倍体分析相对细胞学检查对诊断良恶性浆膜腔积液的灵敏度更高,而这两种方法联合检测则能进一步提高诊断率,表明DNA倍体分析技术可作为细胞学检查的补充,具有重要临床价值。Objective To explore the clinical application value of DNA doublet analysis in diagnose benign and malignant serous cavity effusion. Methods Ninety-five benign and 68 cases of malignant serous effusions were clinically confirmed. Flow cytometry and microscopy were used to analyze the exfoliated cells in each sample for DNA ploidy and morphological examination.Results The sensitivity of DNA ploidy analysis and cytology for the diagnosis of malignant serous effusion were 75% and 42.6%,respectively,and the negative predictive values were 84.5% and 70.9%,respectively,and the difference is statistically significant（P 0.01）;The specificity of DNA ploidy analysis and cytology for the diagnosis of malignant effusion was 97.9% and 100%,respectively. The positive predictive value was 96.2% and the positive predictive value was 100%. The difference was not statistically significant. The combined sensitivity of these two methods was 95.6%,which was significantly higher than the single detection method（P〈0.05）. The combined specificity was 100%. There was no difference compared to the single detection method. Conclusion DNA doublet analysis based flow cytometry is more sensitive to the diagnosis of benign and malignant serous effusions,and combined detection of these two methods can further improve the diagnostic rate,indicating that DNA ploidy analysis technology can be used as The addition of cytology has important clinical value.

关 键 词：浆膜腔积液 DNA倍体分析 流式细胞术 显微镜 

分 类 号：R446.11[医药卫生—诊断学]