一测多评法同时测定肉豆蔻-8散中5种成分  

Simultaneous determination of five constituents in Roudoukou-8 Powder by QAMS

作　　者：贾文婷 张媛彦 郭娜 王玉华 JIA Wen-ting;ZANG Yuan-yan;GUO Na;WANG Yu-hua(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《中成药》2018年第6期1307-1312,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81360678);内蒙古自治区科技计划项目(1405125)

摘　　要：目的建立一测多评法同时测定肉豆蔻-8散(肉豆蔻、木香、沉香等)中5种成分的含有量。方法用75%甲醇提取该药物,方法为采用Apollo C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长225、254、273、281 nm。以丁香酚为内标,建立其他4种成分的相对校正因子,测定其含有量。结果鞣花酸、丁香酚、木香烃内酯、去氢木香内酯和去氢二异丁香酚分别在0.227 0~1.135 2、5.272 2~26.361 0、0.540 8~2.704 0、0.530 4~2.652 0、0.059 0~0.299 5μg范围内线性关系良好(r>0.999 0),平均加样回收率(RSD)分别为96.37%(2.07%)、102.19%(2.78%)、101.66%(1.66%)、103.46%(1.17%)、98.25%(1.98%),一测多评法所得结果与外标法接近。结论该方法稳定可靠,可用于肉豆蔻-8散的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker（ QAMS） method for the simultaneous content determination of five constituents in Roudoukou-8 Powder（ Myristicae Semen,Aucklandiae Radix,Lignum aquilariae Resinatum,etc.）. METHODS The analysis of 75% methanol extract of this drug was performed on a 30 ℃ thermostatic Apollo C_（18） column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of methanol-0. 1% phosphoric acid flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelengths were set at 225,254,273,281 nm. With eugenol as an internal standard,the relative correction factors of the other four constituents were calculated,after which the content determination was made.RESULTS Ellagic acid,eugenol,costunolide,dehydroroma lactone,dehydrodiisoeugenol showed good linear relationships within the ranges of 0. 227 0-1. 135 2,5. 272 2-26. 361 0,0. 540 8-2. 704 0,0. 530 4-2. 652 0,0. 059 0-0. 299 5 μg（ r 〉0. 999 0）,whose average recoveries（ RSDs） were 96. 37%（ 2. 07%）,102. 19%（ 2. 78%）,101. 66%（ 1. 66%）,103. 46%（ 1. 17%）,98. 25%（ 1. 98%）,respectively. The results obtained by QAMS approximated those obtained by external standard method. CONCLUSION This stable and reliable method can be used for the quality control of Roudoukou-8 Powder.

关 键 词：肉豆蔻-8散 鞣花酸 丁香酚 木香烃内酯 去氢木香内酯 去氢二异丁香酚 一测多评 

分 类 号：R927.2[医药卫生—药学]