一株抗烟草赤星病解淀粉芽孢杆菌的筛选及鉴定  

Screening and identification of an antagonistic Bacillus amyloliquefaciens against tobacco brown spot

作　　者：司世飞 宋莉莎 任静[1] 刘仁祥 李忠[1,2] SI Shifei;SONG Lisha;REN Jing;LIU Renxiang;LI Zhong(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Key Laboratoryfor Tobacco Quality Study,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]贵州大学贵州省烟草品质研究重点实验室,贵州贵阳550025

出　　处：《河北大学学报（自然科学版）》2018年第4期410-415,共6页Journal of Hebei University(Natural Science Edition)

基　　金：贵州省烟草公司资助项目(中烟黔科[2014]2号);贵州省烟草品质遗传改良与生物转化科技创新人才团队建设项目(黔科合人才团队[2015]4011)

摘　　要：从土壤中分离出82株细菌,通过平板对峙法初筛和双层平板法复筛,筛选出对烟草赤星病菌有较强拮抗作用的细菌B11菌株,其无菌发酵液对烟草赤星病菌(Alternaria alternata)的抑菌带达到26.55mm.对B11菌株进行生理生化和分子鉴定,结果表明B11菌株生理生化特征与Bacillus amyloliquefaciens最为接近;16SrRNA基因序列分析结果表明该菌株与B.amyloliquefaciens(NR-116022)聚类呈一支,同源性为99%,因此把该菌株鉴定为解淀粉芽孢杆菌(B.amyloliquefaciens).进一步研究表明B11菌株对几种植物病原真菌的生长均有抑制作用.82 bacterial strains were obtained from soil.By plate confrontation method and preliminary screening and double plate screening,strain B11 was screened to have the strongest inhibition against Alternaria alternata.The inhibitory zone of aseptic fermented liquid against A.alternatareached 26.55 mm.Physiological,biochemical and molecular identification of B11 strain were carried out.The physiological and biochemical characteristics of B11 strain were closest to that of Bacillus amyloliquefaciens.16 SrRNA sequence analysis of strain B11 showed that B.amyloliquefaciens（NR-116022）clustering and strain B11 became a branch（99% similarity）.So,it was identified as B.amyloliquefaciens.Further studies showed that B11 strain had some inhibitory effects on several plant pathogenic fungi.

关 键 词：烟草赤星病菌 解淀粉芽孢杆菌 鉴定 抑菌作用 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]