秦岭滑蜥视网膜年龄相关的显微结构特征和胶质纤维酸性蛋白免疫组织化学定位  

Age-dependent Microstructure and Immunohistochemical Localization of Glial Fibrillary Acidic Protein in the Retina of Scincella tsinlingensis

作　　者：高燕燕 李琳 赵禹 朱妞 杨纯[1] GAO Yanyan;LI Lin;ZHAO Yu;ZHU Niu;YANG Chun(School of Life Sciences,Shanxi Normal University,Linfen,Shanxi Province 041004,China)

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《四川动物》2018年第4期439-444,共6页Sichuan Journal of Zoology

基　　金：国家级大学生创新创业训练计划项目(201710118002)

摘　　要：光学显微镜下观察了秦岭滑蜥Scincella tsinlingensis视网膜年龄相关的显微结构特征,并对胶质纤维酸性蛋白(GFAP)免疫组织化学定位及表达强度进行了统计分析。结果显示:秦岭滑蜥视网膜由内至外共分为10层,与昼行性蜥蜴类视网膜结构特征一致。亚成体和成体视网膜总厚度较幼体明显增加,但三者神经节细胞层、外界膜以及视杆视锥层厚度之间无明显差异。不同年龄组视网膜细胞核层数无差异:外核层1~2层,神经节细胞层3~4层,内核层6~8层。视网膜GFAP免疫阳性主要定位于内界膜和神经纤维层,且神经纤维层阳性最强。GFAP阳性表达呈现成体最强、幼体较强、亚成体最弱的年龄相关性,这可能与视网膜胚后发育中的生理功能调节相关。The age-dependent microscopic structure of Scincella tsinlingensis retina was observand under observd under light microscope and the immunohistochemical localization and expression intensity of glial fibrillary acidic protein （GFAP） were statisticallyanalyzed. The results showed that the retina of S. tsinlingensis was divided into 10 layers from the inside to outside, and thiswas consistent with the structural characteristics of lizards’ retina. The total thickness of sub-adult and adult retina increasedsignificantly compared with the juvenile, however, no significant difference was observed a m ong the thickness of ganglion cells layer, outer limiting membrane, and layer of rods and cones. There were also no significant differences in the number of retinal nucleus layers among different age groups, which were composed of 1 - 2 layers in the outer nuclear layer,3-4 layers in the ganglion cells layer and 6-8 layers in the inner nuclear layer. The immunoreactivity of GFAP in the reti-na was mainly located in the inner limiting membrane and the nerve filament layer, and the nervv fiber layer was the most positive. The expression of GFAP in the retina showed age-related changes： adult 〉 juvenile 〉 subadult. Therefore, the ex-pression of GFAP in the retina of S. tsinlingensis is related to age, and this may be involved in the regeylation of physiological functions during the development of retina.

关 键 词：秦岭滑蜥 视网膜 胶质纤维酸性蛋白 

分 类 号：Q954[生物学—动物学] Q959.6