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作 者:侯典云[1,2] 杨萌萌 周爽 赵杏利[1,2] 张红晓[1,2] 胥华伟 HOU Dian-yun;YANG Meng-meng;ZHOU Shuang;ZHAO Xing-li;ZHANG Hong-xiao;XU Hua-wei(College of Agriculture,Henan University of Science and Technology;The Luoyang Engineering Researeh Center of Breeding and Utilization of Dao-di Herbs,Luoyang 471023,China)
机构地区:[1]河南科技大学农学院 [2]洛阳市道地药材繁育与创新利用工程技术研究中心,洛阳471023
出 处:《天然产物研究与开发》2018年第7期1126-1131,共6页Natural Product Research and Development
基 金:国家自然科学基金(U1404829)
摘 要:本文以獐牙菜属的六种药材为研究对象,采用MEGA 6.0软件确定ITS2序列的GC含量,基于K2P模型计算遗传距离,构建NJ树,ITS2 database在线分析二级结构等方法,研究ITS2条形码序列对六种药材的鉴别效果。结果表明,六种药材的ITS2序列长度范围为230~232 bp,平均GC含量为61.2%~64.0%。六种药材的种内变异均较小,其种间平均遗传距离为0.03~0.09。ITS2二级结构的茎环结构和四个臂均不同,NJ树结果显示,六种药材可分别聚为一支,呈现出明显的单系性。因此,ITS2序列可准确、有效地鉴别本研究的獐牙菜属六种药材,为这些药材的后续相关深入研究奠定了基础,也为獐牙菜属其他物种鉴定提供了参考。To identify the six medicinal materials in Swertia. by ITS2 sequences. Total genomic DNA from all the samples were extracted using the Plant Genomic DNA kit. The ITS2 regions were amplified,bidirectional sequenced and aligned.The average GC content was analyzed through MEGA 6. 0 software. The intra-versus inter-specific genetic distances of the ITS2 regions were computed in accordance with the kimura 2-parameter( K2 P) model and Neighbor-joining( NJ)phylogenetic trees were constructed using MEGA6. 0. Secondary structure has been analyzed through online ITS2 database. The length of ITS2 from six medicinal materials in Swertia varied in 230-232 bp and average GC content was61. 2%-64. 0%. The intraspecific variation of six medicinal materialswas small respectively. The intraspecific variation of S. delavayi was largest,and the average intraspecific genetic distance was 0. 02. However,the average interspecific genetic distance of the six medicinal materials in Swertiawas 0. 03-0. 09. The results of secondary structure of ITS2 showed that the stem-and-loop structure and four arms of ITS2 from these samples have significant difference. NJ trees can identify the six medicinal materials,which present the obvious monophyly. ITS2 regions can identify the six medicinal materials in Swertia effectively and accurately. Which lay a foundation for the relevant in-depth study of these six medicinal materials,and provide an important reference as the identification of others Swertia.
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