广藿香内生真菌Daldinia eschscholtzii A630次级代谢产物及其细胞毒活性研究  

Secondary Metabolites of Endophytic Daldinia eschscholtzii A630 from Pogostemon cablin and Their Cytotoxic Activities

作　　者：卢梦梦[1,2] 陈玉婵[1] 刘洪新[1] 李赛妮 李浩华[1] 陶美华[1] 郝再彬[2] 章卫民[1] LU Meng-meng;CHEN Yu-chan;LIU Hong-xin;LI Sai-ni;LI Hao-hua;TAO Mei-hua;HAO Zai-bin;ZHANG Wei-min(State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China;College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541006,China)

机构地区：[1]广东省微生物研究所省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广州510070 [2]桂林理工大学化学与生物工程学院,桂林541006

出　　处：《天然产物研究与开发》2018年第7期1176-1180,共5页Natural Product Research and Development

基　　金：国家自然科学基金(31600271); 国家973前期专项(2014CB460613); 广东省科技计划(2015A030302060,2014A030304050); 广东省自然科学基金(2015A030313710)

摘　　要：采用色谱法从广藿香内生真菌Daldinia eschscholtzii发酵液中分离得到9个化合物,通过波谱数据分析分别鉴定为dihydrosphaerolone(1)、2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one(2)、6-hydroxymellein(3)、4,8-dihydroxy-1-tetralone(4)、8-methoxy-1-naphthol(5)、helicascolide A(6)、1-(2,6-dihydroxyphenyl)butan-1-one(7)、1-(2,6-dihydroxyphenyl)ethan-1-one(8)、diisobutyl phthalate(9),其中化合物5、9为首次从该属真菌中分离得到。细胞毒活性测试显示化合物6、7对肿瘤细胞SF-268、MCF-7、NCI-H460、Hep G-2具有较好的抑制活性,IC50值在16.52~28.42μmol/L之间。Nine compounds were isolated from the fermentation broth of endophytic fungus Daldinia eschscholtzii from Pogostemon cablin by using various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH-20,preparative TLC and HPLC. Their structures were identified as dihydrosphaerolone（ 1）,2,3-dihydro-5-hydroxy-2-methyl-4 H-1-benzopyran-4-one（ 2）,6-hydroxymellein（ 3）,4,8-dihydroxy-1-tetralone（ 4）,8-methoxy-1-naphthol（ 5）,helicascolide A（ 6）,1-（ 2,6-dihydroxyphenyl）-butan-1-one（ 7）,1-（ 2,6-dihydroxyphenyl） ethan-1-one（ 8）,diisobutyl phthalate（ 9）,respectively,based on spectroscopic analysis. Among them,compounds 5 and 9 were isolated from the genus Daldinia for the first time. Compounds 6 and 7 showed moderate growth-inhibitory activities in vitro against SF-268,MCF-7,NCI-H460 and Hep G-2 tumor cell lines with IC50 values ranging from 16. 52 to 28. 42 μmol/L.

关 键 词：广藿香 内生真菌 Daldinia eschscholtzii 次级代谢产物 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学] Q93-3[医药卫生—中医学]