RP-HPLC法测定不同产地及不同部位马蓝中六种活性成分的含量  

Determination Analysis of Six Bioactive Constituents Different Parts in Different Habitats of Baphicacanthus cusia( Nees) Bremek by RP-HPLC

作　　者：肖春霞 杨万霞[2] 涂江涛 苑春茂[2] 黄烈军[2] 胡永 段玉书 顾玮[2] 郝小江[2,3] XIAO Chun-xia;YANG Wan-xia;TU Jiang-tao;YUAN Chun-mao;HUANG HU Yong;DUAN Yu-shu;GU Wei;HAO Xiao-jiang(College of pharmacy,Guizhou Urliversity,Guiyang 550025,China;State Key Laboratory of Functions and Applications of Medicinal Plants,The Key Laboratory of Chemistry jbr Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550000,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China)

机构地区：[1]贵州大学药学院,贵阳550025 [2]省部共建药用植物功效与利用国家重点实验室,贵州省中国科学院天然产物化学重点实验室,贵阳550000 [3]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650201

出　　处：《天然产物研究与开发》2018年第7期1188-1194,共7页Natural Product Research and Development

基　　金：贵州省自然科学基金(黔科合J字[2015]2105号); 国家自然科学基金(31600280)

摘　　要：建立了RP-HPLC法测定马蓝中色胺酮、2-苯并噁唑酮、靛玉红,靛蓝、阿克苷和角胡麻苷六种活性成分含量的方法,并对不同产地及不同部位药材含量进行了分析。采用ZORBAX SB-C18色谱柱(4.6×250 mm,5μm),以乙腈和水为流动相梯度洗脱,流速为1 m L/min;检测波长280 nm;柱温30℃。马蓝中六种化学成分分离良好,各成分含量与峰面积在测定范围内均呈良好的线性关系(r≥0.999 1),加样回收率分别为100.25%、99.93%、100.46%、99.94%、99.92%、100.02%,RSD值分别为1.46、0.13、0.52、0.09、0.16、0.40。该方法简便快捷、重现性好,可为南板蓝根药材的品质评价和质量控制标准提供科学依据。This research aims to establish a RP-HPLC method for analysis of bioactive constituents such as tryptanthrin,2（ 3 H）-benzoxazolinone,indirubin,indigo,acteoside,martynoside of different parts of Baphicacanthus cusia（ Nees） Bremek in different habitats. The chromatographic separation was performed on ZORBAX SB-C18 column（ 4. 6 × 250 mm,5μm） with gradient elution consisting of acetonitrile and water at a flow rate 1 m L/min. The UV detection wavelength was280 nm and the column temperature was set at 30 ℃. The six kinds of compounds in B. cusia separated well,and the linear relation was obtained（ r ≥ 0. 999 1）. The average recoveries were 100. 25%,99. 93%,100. 46%,99. 94%,99. 92% and 100. 02%,with RSD 1. 46,0. 13,0. 52,0. 09,0. 16 and 0. 40. The method is simple,quick,and good reproducibility,which can provide the basis for the rational use of the active parts of B. cusia.

关 键 词：含量测定 马蓝 RP-HPLC 不同产地 不同部位 

分 类 号：R927.2[医药卫生—药学] Q946[生物学—植物学]