病毒拟似物Poly(I：C)诱导人卵巢颗粒细胞病毒反应蛋白viperin表达  

Viperin is induced by Poly(I: C) stimulation in human ovarian granulosa cells

作　　者：王波 吴英[3] 方玉莹[4] 陈丹青 吕时铭[2] 高玉平[1] WANG Bo;WU Ying;FANG Yu-ying;CHEN Dan-qing;LYU Shi-ming;GAO Yu-ping(Xinhua Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200092;the Second Affiliated Hospital of Xinjiang Shihezi University（Corps Hospital）,Urumqi 830000;Shanghai First Maternity and Infant Hospital,Shanghai 201204;Women＇s Hospital,School of Medicine,Zhejiang University,Hangzhou 310006,China)

机构地区：[1]上海交通大学医学院附属新华医院,上海200092 [2]浙江大学医学院附属妇产科医院,浙江杭州310006 [3]新疆石河子大学第二附属医院(兵团医院),新疆乌鲁木齐830000 [4]上海市第一妇婴保健院,上海201204

出　　处：《基础医学与临床》2018年第8期1069-1073,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81070535);上海市卫生和计划生育委员会科研课题(201640185);教育部生殖遗传重点实验室主任基金(2012-RG-BF-03);浙江省生殖医学重点科技创新团队自选项目(2011R50013-03)

摘　　要：目的探讨病毒拟似物对人卵巢颗粒细胞病毒反应蛋白表达的影响,明确颗粒细胞在卵巢病毒感染时的反应。方法将原代培养的人卵巢颗粒细胞分为对照组、病毒拟似物Poly(I:C)处理组、卵泡刺激素(FSH)处理组。CCK8法测定细胞增殖活力、real-time PCR法测定干扰素-β(IFN-β)、viperin mRNA表达,Western blot法测定viperin蛋白表达。结果原代接种培养5 d后,FSH可诱导颗粒细胞增殖(P<0.05),Poly(I:C)10μg/m L对细胞增殖活力无显著影响。Poly(I:C)刺激后24 h内诱导IFN-β、viperin mRNA表达明显上调,其中IFN-β于刺激后2 h表达水平达到峰值(P<0.05),viperin于刺激后8 h达到峰值(P<0.05),刺激后24 h,两者mRNA表达水平均回归刺激前水平。Poly(I:C)诱导颗粒细胞viperin蛋白表达,水平随Poly(I:C)浓度上升。结论病毒拟似物能够诱导颗粒细胞表达病毒反应蛋白viperin。Objective To investigate the effect of Poly（ I： C） on the expression of a virus responsive protein,viperin. Methods Human granulosa cells were obtained from patients with in vitro fertilization. After incubation with FSH or Poly（ I： C）,the proliferation of granulosa cells was detected by CCK8 method. The expression of viperin and IFN-β were examined by real time PCR after a certain period of time after Poly（ I： C） stimulation within 24 hours. The protein expression of viperin was examined by Western blot analysis. Results 10 μg/m L of Poly（ I： C） has no significant influence on proliferation of granulosa cells. Poly（ I： C） significantly induced mRNA expression of IFN-β（ 2 to 4 hours after stimulation） and viperin（ 8 to 12 hours after stimulation）（ P〈0. 05）.Viperin expression was induced by Poly（ I： C） in a dose-dependent manner. Conclusions Poly（ I： C） induces IFN-β and viperin expression in human granulosa cells,which indicates that granulosa cells play an active role in anti-virus response upon infection.

关 键 词：颗粒细胞 viperin 病毒感染 干扰素-Β 

分 类 号：R711.3[医药卫生—妇产科学]