两种实验性腹主动脉瘤大鼠模型的比较  

Comparison on the Two Experimental Models of Abdominal Aortic Aneurysm in Rats

作　　者：吴建秋[1] 景在平[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中国动脉硬化杂志》2002年第4期277-280,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 39770 72 1)资助

摘　　要：为探讨和比较两种腹主动脉瘤大鼠模型特征 ,以便进一步为腹主动脉瘤体内研究提供基础 ,选取SD大鼠肾下腹主动脉猪胰腺弹力蛋白酶恒压灌注 ,或使用豚鼠肾下腹主动脉正位替换。利用特殊染色和免疫组化技术 ,分别于术后 2周和 4周 ,观察腹主动脉形成率、形态学变化及基质金属蛋白酶水平。结果发现 ,6 4%酶灌注大鼠和 88%异种移植大鼠形成腹主动脉瘤 ,均无出血或腔内血栓形成。与灌注型腹主动脉瘤相比 ,移植型腹主动脉瘤壁损伤更为严重 ,基质金属蛋白酶 2及基质金属蛋白酶 9表达也显著增高。证明两种方法均能诱导腹主动脉瘤形成 。Aim To compare and contrast two kinds of abdominal aortic aneurysm(AAA) model of rats, and thus to establish the foundation for the further study of AAA in vivo. Methods Commercially available porcine pancreatic elastase was Infused into the SD rat abdominal aorta, or orthotopic engraftment of guinea pig aorta was transplanted into the SD rat abdominal aorta. The patency, and macroscopic, microscopic morphologic and matrix metalloproteinase(MMP) changes that occurred in xenografts and the controls were compared postoperative 2 weeks or 4 weeks. Results There were AAA formation in 64 percent infused rats and 88 percent transplanted rats, without haemorrhage and intraluminal thrombus. In comparison to the AAA induced by elastase, the lesions of AAA walls after xenograft were more serious and the expression of MMP-2 and MMP-9 were remarkably increased. Conclusions Both of the methods may induce AAA,but xenograft model has higher stability and reliability.

关 键 词：腹主动脉瘤 大鼠 金属蛋白酶 实验研究 

分 类 号：R543.16[医药卫生—心血管疾病]