腹主动脉瘤发病中平滑肌细胞增殖与凋亡的关系  

Relation between Vascular Smooth Muscle Cell Proliferation and Apoptosis during Abdominal Aortic Aneurysm Formation

作　　者：吴建秋[1] 景在平[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中国动脉硬化杂志》2002年第4期281-284,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 39770 72 1)资助

摘　　要：探讨在腹主动脉瘤形成过程中细胞周期调控下的细胞增殖与死亡的关系。选取豚鼠—大鼠异种移植腹主动脉瘤模型 ,以平滑肌细胞α 肌动蛋白、增殖细胞核抗原抗体免疫组织化学检测和原位末端DNA标记法分别作为血管平滑肌细胞定性定位、细胞增殖与凋亡分析方法 ,利用计算机图像分析技术动态观测术后 1、2、3、4周阳性细胞灰度值和百分比。结果发现 ,1～ 2周 ,移植腹主动脉直径已有中度扩张 ,细胞增殖指数大于细胞凋亡指数 ;3～4周 ,移植物呈显著扩张 ,细胞凋亡指数大于细胞增殖指数 ;移植组血管平滑肌细胞凋亡与移植动脉直径呈现显著正相关。证明细胞增殖和凋亡贯穿于腹主动脉损伤重构的全过程 。Aim To assess the corelation between vascular smooth muscle cell proliferation and apoptosis, under the regulation of cell cycle, during abdominal aortic aneurysm formation and development. Methods Guinea pig-SD rat abdominal aorta xenografts were used as experimental model. Immunohistochemistry and in situ end-labeling of DNA fragments determined vascular smooth muscle cell and their proliferation and apoptosis. Cell proliferation and apoptosis index were counted with the use of computer-generated image analysis at 1,2,3,4 week after transplantation. Results Graft dilation, during 1-2 weeks, were moderate, but marked in the later 2 weeks in comparison to the controls. Vascular smooth muscle cell proliferation proceeds apoptosis by 2 weeks, and shifts to the contrary in another 2 weeks. The abdominal aortic diameter had a significant correlation with increasing vascular smooth muscle cell apoptosis in xenograft. Conclusion vascular smooth muscle cell proliferation and apoptosis is connected with abdominal aortic structure damage and remodeling, whose imbalance contributes to abdominal aortic aneurysm formation and development.

关 键 词：腹主动脉瘤 平滑肌细胞增殖 发病 细胞凋亡 实验研究 

分 类 号：R543.16[医药卫生—心血管疾病]