电晕场处理与介电分选对杂交水稻种子活力的影响  

Effect of corona treatment and dielectric separation on seed vigor of hybrid rice

作　　者：庞嘉[1] 张海清[1] 刘爱民 贺记外 刘烨[1] 刘俊龙[1] 张艺馨 刘虹 PANG Jia;ZHANG Haiqing;LIU Aimin;HE Jiwai;LIU Ye;LIU Junlong;ZHANG Yixin;LIU Hong(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Longping Seed Co.Ltd.,Changsha,Hunan 410006,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]湖南隆平种业有限公司,湖南长沙410006

出　　处：《湖南农业大学学报（自然科学版）》2018年第4期339-345,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家科技支撑计划项目(2014BAD06B07);2016年省级大学生创新性实验计划项目(SCX1634)

摘　　要：2017年,以2015、2016年生产的N两优华占、望两优6号种子以及2014、2016年生产的Y两优9918种子为试材,对杂交水稻种子进行电晕场处理、介电分选及电晕场+介电分选处理,通过测定种子的活力及部分生理生化指标,以筛选适宜的电晕场与介电分选处理。结果表明:当场强200 k V/m,处理时间4 min时,种子活力显著提高,其中Y两优9918种子(2014年产)发芽势和发芽率比对照分别提高47.22%和23.53%;电晕场处理使种子的电导率及幼苗的丙二醛(MDA)含量均降低,脯氨酸含量与过氧化物酶(POD)活性均升高,根系活力增强,且电导率与对照的差异达显著水平;当滚筒转速为30 r/min,介电分选电压为4 500 V时,分选效果较好,各级种子活力差异显著;电晕场优化处理结合介电分选优化电压处理,分选的各级种子活力均比单独介电分选明显提高,其中望两优6号(2016年产)组合处理比单独介电分选出的Ⅰ级、Ⅱ级和Ⅲ级种子发芽势分别提高14.28%、18.42%和15.52%,发芽率分别提高7.78%、9.64%和14.71%。In 2017, N Liangyouhuazhan and Wangliangyou 6 hybrid seeds producted in 2015 and 2016, and Y Liangyou9918 hybrid seeds producted in 2014 and 2016 were used as materials, in this study, treatments of corona field, the dielectric separation and the corona field＋dielectric separation on hybrid rice seeds were conducted, seed vigor and several physiological and biochemical indicators were measured to screen the suitable corona field and dielectric separation. The results showed that the seed vigor increased significantly under 200 k V/m corona field for 4 min. The seed germination potential and germination rate of Y Liangyou 9918（2014） were 47.22% and 23.53% higher than the control, respectively; The seed conductivity and the content of malondialdehyde in seedling were reduced, but the content of proline, activity of peroxidase and root vitality were increased after the seeds treated by corona field; The sorting seeds effect of seed separation with parameters of 30 rotation per min and 4 500 voltage is preferably, and the vigor of different levels seeds that sorted by using these parameters showed significant difference; The vigor of different grades seed that separated from initial seeds by using corona field combined with the dielectric separation is significantly higher than that treated only by the corona field or the dielectric separation, among the hybrid seeds treated by corona field combining with the dielectric separation, the seed vigor of Wangliangyou 6（2016） was the highest, compared with those that treated only by the corona field or the dielectric separation single germination potentials of class I, class II and class III seeds increased by 14.28, 18.42 and 15.22 percentage points, respectively; the germination rate increased by 7.78, 9.64 and14.71 percentage points, respectively.

关 键 词：杂交水稻 电晕场处理 介电分选 种子活力 

分 类 号：S511[农业科学—作物学]