磨盘草乙酸乙酯部位的化学成分分析  

Chemical constituents of ethyl acetate fraction from Abutilon indicum(L.) Sweet

作　　者：张昕 魏江存 阙祖亮 李耀华 陈勇 ZHANG Xin;WEI Jiang-cun;QUE Zu-liang;LI Yao-hua;CHEN Yong(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,Chin)

机构地区：[1]广西中医药大学药学院,南宁市530200

出　　处：《广西医学》2018年第14期1585-1590,共6页Guangxi Medical Journal

基　　金：国家自然科学基金(81260673;81660701);广西自然科学基金(2016GXNSFAA380148;2014GXNSFAA118208);广西研究生教育创新计划(YJSP201712);广西高校中药提取纯化与质量分析重点实验室(桂教科研[2014]6号);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室(国中医药发2009[21]号)

摘　　要：目的分析磨盘草乙酸乙酯部位的化学成分。方法取磨盘草制成乙醇浸膏,经石油醚、乙酸乙酯萃取后得到乙酸乙酯部位浸膏,利用硅胶柱色谱及液相-质谱联用技术分析磨盘草乙酸乙酯部位中的化学成分。结果通过硅胶柱色谱分离并鉴定出了2个化合物,分别为β-谷甾醇、齐墩果酸;通过液相-质谱联用技术鉴定出了9个化合物,分别为对羟基苯甲酸、对羟基反式肉桂酸、阿魏酰酪胺、硬脂酰胺、二十二烷酰胺、东莨菪内酯、异嗪皮啶、8-羟基-5,6,7-三甲氧基香豆素、棕榈酰胺。结论对磨盘草乙酸乙酯部位进行化学成分分析可为磨盘草的深入研究提供参考。Objective To analyze the chemical constituents of the ethyl acetate fraction from Abutilon indicum（ L.） Sweet.Methods Abutilon indicum（ L.） Sweet was made into ethanol extractum,then extractum with ethyl acetate fraction was obtained after extraction by aether petrolei and ethyl acetate. Silica gel column chromatography and liquid chromatography-mass spectrometry（ LC-MS）were used to analyze the chemical constituents of ethyl acetate extract of Abutilon indicum（ L.） Sweet. Results Two compounds,β sitosterol and oleanolic acid,were isolated and identified by silica gel column chromatography from ethyl acetate extract. Nine compounds were identified by LC-MS,including paracyanobenzoic acid,ρ-coumaric acid,feruloyltyramine,stearic amide,docosanamide,isofraxidin,8-hydroxy-5,6,7-trimethoxycoumarin and palmitamide. Conclusion The chemical composition analysis of the ethyl acetate fraction of Abutilon indicum（ L.） Sweet can provide a basis for the in-depth study of Abutilon indicum（ L.） Sweet.

关 键 词：磨盘草 乙酸乙酯部位 化学成分 硅胶柱色谱 液相-质谱联用技术 

分 类 号：R284.1[医药卫生—中药学]