山西地区仔兔腹泻源大肠杆菌的分离·鉴定及耐药性分析  

Separation,Identification and Drug Resistance Analysis of Escherichia coli from Baby Rabbits with Diarrhea in Shanxi Area

作　　者：王国艳[1] 孙乐天[2] 张国权[1] 张晋强[1] 冯国亮[1] 曹亮[1] 任克良[1] WANG Guo-yan1, SUN Le-tian2, ZHANG Guo-quan1(1.Institute of Animal Husbandry and Veterinary Sciences, Shanxi Academyof Agricultural Sciences,Taiyuan, Shanxi 030032; 2.Taiyuan Zoo, Taiyuan, Shanxi 03000)

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032 [2]太原动物园,山西太原030009

出　　处：《安徽农业科学》2018年第22期69-70,77,共3页Journal of Anhui Agricultural Sciences

基　　金：国家兔产业技术体系专项(CARS-43-B-3);山西省农业科学特色农业技术攻关项目(YGG17092)

摘　　要：[目的]调查山西地区仔兔腹泻源致病性大肠杆菌的耐药性。[方法]从山西地区不同兔养殖场采集110份因腹泻死亡的仔兔肠道内容物,进行病原分离,并通过菌落形态观察和生化试验对其进行鉴定。开展动物致病性试验,最后采用K-B纸片法用14种抗生素对分离菌株进行药物敏感性测定。[结果]共分离出60株细菌,通过菌落形态观察和生化试验均鉴定为大肠杆菌。药敏试验结果表明,分离的60株大肠杆菌对阿米卡星的耐药率为0,而对庆大霉素、链霉素、多粘菌素B、卡那霉素、氧氟沙星、诺氟沙星、痢特灵、头孢克罗、阿莫西林、头孢曲松、头孢哌酮、氯霉素、磷霉素13种常见药物均表现出不同程度的耐药性,其中对头孢曲松、磷霉素、多粘菌素B、阿莫西林、痢特灵、氯霉素、头孢哌酮的耐药率均在80%以上;分离菌株对卡那霉素的耐药率最低,为3.3%。[结论]山西地区仔兔腹泻源致病性大肠杆菌对13种抗生素产生了普遍的耐药性,建议进一步扩大药物的筛选范围,为大肠杆菌病的防制和临床用药的风险评估提供科学依据。[Objective] To investigate the drug resistance of pathogenic Escherichia coli derived from baby rabbits with diarrhea in Shanxi area.[Method] 110 samples of intestine contents of diarrhea-infected rabbits were collected from different rabbit farms of Shanxi to separate patho-gen. And the pathogen was identified by colony characteristics observation and biochemical test. The animal pathogenicity test was made andthe drug sensitivity of isolated bacteria to 14 kinds of antibiotics were determined by using K-B disk diffusion method. [Result] 60 strains ofpathogen were isolated and identified as E. coli by colony characteristics observation and biochemical test.The results of drug sensitivity testshowed that the drug resistance rate of isolated strains to amikacin was 0,and isolated strains showed different drug resistance to 13 kinds of an-tibiotics（gentamicin, streptomycin, polymyxin B, kanamycin, ofloxacin, norfloxacin, furazolidone, cefaclor, amoxicillin, ceftriaxone, cef-operazone, chloramphenicol and fosfomycin）. The drug resistance rate of isolated strains to ceftriaxone, fosfomycin, polymyxin B, amoxicillin,furazolidone, chloramphenicol and cefoperazone were all above 80%. The drug resistance rate of isolated strains to kanamycin was the lowest,being 3.3%. [Conclusion] The pathogenic E. coli derived from baby rabbits with diarrhea in Shanxi area had common drug resistance to 13kinds of antibiotics,therefore it was suggested that the screening scope of drugs should be further expanded ,so as to provide scientific basis forthe prevention and control of rabbit colibacillosis and clinical drug’s risk assessment.

关 键 词：仔兔 腹泻 大肠杆菌 耐药性 

分 类 号：S855[农业科学—临床兽医学]