VEGF基因单核苷酸多态性与习惯性流产患者的相关性研究  

Correlation between VEGF gene single nucleotide polymorphism and recurrent spontaneous abortion

作　　者：罗莉[1] 暴蕾[1] 吕海荣[1] 赵静[1] LUO Li, BAO Lei,LV Hairong,et al(The Fourth Hospital of Xi＇ an City,Shaanxi,Xi＇ an 710004,Chin)

机构地区：[1]陕西省西安市第四医院,710004

出　　处：《河北医药》2018年第16期2405-2410,共6页Hebei Medical Journal

基　　金：陕西省科技攻关项目(编号:2014K11-03-09-04)

摘　　要：目的对血管内皮生长因子(vascular endothelial growth factor,VEGF)基因进行了系统扫描,探讨该基因13个单核苷酸多态性位点(single nucleotide polymorphism,SNP)与习惯性流产(recurrent spontaneous abortion,RSA)的相关性。方法选择了VEGF基因13个位点通过基质辅助激光解吸电离飞行时间质谱技术对227例RSA患者和232例健康对照进行了基因分型及数据统计分析。结果 RSA组及对照组VEGF基因13个位点基因频率分布符合H-W平衡。VEGF基因发现3个block处于强连锁不平衡(D’>0.9)。RSA组rs3025020位点T等位基因频率显著高于对照组(P<0.05);RSA组rs3025039位点C等位基因频率显著低于对照组(P<0.05)。在block3中,对照组中CG-C单倍型频率显著高于病例组(P<0.05);病例组中T-G-C单倍型频率显著高于对照组(P=0.05)。结论 VEGF基因rs3025020(Intron 6)与rs3025039(3’UTR)位点多态性可能与RSA有关,携带有rs3025020多态性位点T等位基因与rs3025039多态性位点T等位基因的个体可能更容易患RSA,携带有T-G-C单倍型个体可能更容易患RSA。Objective To scan the functional SNPs of vascular endothelial growth factor（ VEGF） gene,and to investigate the correlation between VEGF single nucleotide polymorphism（ SNP） and recurrent spontaneous abortion（ RSA）.Methods The genotyping and data of VEGF gene in 13 locus were analyzed by Mass ARRAY system in 227 cases of RSA and232 helthy subjects. Results The distribution frequencies of 13 genotype SNPs of VEGF were accordant with Hardy-Weinberg equilibrium. The analysis of VEGF gene showed that there were 3 blocks in strong linkage disequilibrium（ D＇ 0. 9）. The RSA patients had a significantly higher frequency of T allele frequency of rs3025020（ χ2= 12. 639,P = 12. 639,OR = 0. 621,95% CI = 0. 477 0. 808）. The healthy subjects had a significantly higher frequency C allele frequency ofrs3025039（ χ2=9. 726,P = 0. 002,OR = 0. 606,95% CI = 0. 442 - 0. 832）. The frequency of C-G-C（ rs3025020-rs3025030-rs3025039）haplotype in control group was significantly higher than that in case group（ P〈0. 01）,moreover,the frequency of T-G-C haplotype in case group was significantly higher than that in control group（ P〈0. 05）. Conclusion VEGF gene rs3025020（ Intron 6） and rs3025039（ 3＇UTR） polymorphism may be correlated with RSA. The subjects with rs3025020 T allele and rs3025039 T allele may be more likely to suffer from RSA,moreover,the subjects with haplotype of T-G-C（ rs3025020-rs3025030-rs3025039） may be more likely to suffer from RSA.

关 键 词：习惯性流产 血管内皮生长因子 单核苷酸多态性 

分 类 号：R714.21[医药卫生—妇产科学]