比色法辅助下紫外分光光度法测定黄芪总黄酮含量  

Determination of Total Flavonoids in Scutellaria Baicalensis by Ultraviolet Spectrophotometry Assisted by Colorimetry

作　　者：陈凌霆 赵娅敏[2] 齐燕姣[1,2] 罗兴平[1,2] 康淑荷[1,2] 张丽丽 俞布仁 杨文亮 Chen Lingting 1,2 ,Zhao Yamin 1,2 , Qi Yanjiao 1,2 , Luo Xingping 1,2 ,Kang Shuhe 1,2 ,Zhang Lili 1,2 ,Yu Buren 3,Yang Wenliang 1,2(1.School of Chemical Engineering, Northwest Minzu University,Lanzhou 730000,China;2.Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass in Universities of Gansu Province,Northwest Minzu University,Lanzhou 730000, China;3.School of Electrical Engineering,Northwest Minzu University,Lanzhou 730000,Chin)

机构地区：[1]西北民族大学化工学院,甘肃兰州730000 [2]西北民族大学甘肃省高校环境友好复合材料及生物质利用省级重点实验室,甘肃兰州730000 [3]西北民族大学电气工程学院,甘肃兰州730000

出　　处：《山东化工》2018年第14期75-77,共3页Shandong Chemical Industry

基　　金：中央高校基本科研业务专向资金项目(31920180003)

摘　　要：目的:建立一种简单、快速、有效的方法测定黄芪中总黄酮含量。方法:在NaNO_3-Al(NO_3)_3-NaOH比色法辅助下,用紫外分光光度法测定黄芪中总黄酮的含量。结果:显色法最佳条件为5%NaNO_3-8%Al(NO_3)_3-5%NaOH,最大吸收波长为512nm,吸光度A与芦丁在0.015~0.6mg/mL范围内呈良好的线性关系(R^2=0.9995),线性方程为A=0.0741c+0.0005,总黄酮的回收率为97.6%,相对偏差(RSD)为2.47%。结论:该法简单准确,易操作,回收率高,可作为黄芪总黄酮含量测定的一种方法。Objective：To establish a simple,rapid and effective method for determination of total flavonoids in Astragalus membranaceus.Methods：The content of total flavonoids in Astragalus membranaceus was determined by UV spectrophotometry with the aid of Na NO3-Al（NO3）3-Na OH colorimetric method.Results：The optimal conditions for chromogenic assay were 5%Na NO3-8%Al（NO3）3-5%Na OH,the maximum absorption wavelength was 512 nm,and the absorbance A was in a good linear relationship with rutin in the range of 0.015-0.6 mg/m L（R^2）.=0.9995）,the linear equation is A=0.0741c＋0.0005,the recovery of total flavonoids was 97.6%,and the relative deviation（RSD）was 2.47%.Conclusion：This method is simple and accurate,easy to operate,and has a high recovery rate.It can be used as a method for the determination of total flavonoids in Scutellaria baicalensis.

关 键 词：黄芪 总黄酮 比色法 紫外分光光度法 

分 类 号：O657.32[理学—分析化学]