星点设计-响应曲面法优化金钗石斛多糖提取工艺  

Optimal Extraction of Polysaccharide from Dendrobium nobile by Central Composite Design-Response Surface Methodology

作　　者：曾宇馨 贺雨馨 祝天添 张晓敏[1] 孙志蓉[1] ZENG Yuxin;HE Yuxin;ZHU Tiantian;ZHANG Xiaomin;SUN Zhirong(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China)

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中医药信息》2018年第3期47-51,共5页Information on Traditional Chinese Medicine

基　　金：贵州赤水石斛产业科技合作项目(No.赤科合02号);现代农业产业技术体系建设专项资金资助(No.CARS-21)

摘　　要：目的:利用星点设计-响应曲面法对金钗石斛多糖提取工艺进行优化研究。方法:在单因素试验的基础上,自变量为金钗石斛的提取温度、提取时间、料液比,因变量为多糖得率,通过对自变量各水平的多元化线性回归及二项式拟合,采用星点设计-响应面法选取金钗石斛多糖提取最佳工艺,并进行预测分析。结果:金钗石斛多糖最优提取工艺条件为:提取温度为65℃,料液比为1∶45,提取时间为72 min,提取次数为2次。提取率预测值为7.49%,实际平均提取率为7.57%,相对误差为0.8%。结论:星点设计-响应曲面法优化金钗石斛多糖提取工艺简便合理,稳定性高,精确度高,可预测性较优。Objective： To optimize polysaccharide extraction from Dendrobium nobile by central composite design - response surface methodology. Mothods： On the basis of single factor experiment, the extraction time,extraction temperature, and material - liquid ratio of Dendrobium nobile were taken as independent variables,polysaccharides was taken as the dependent variables, central composite design - response surface methodologywas used to select the optimum extraction process of polysaccharide from Dendrobium nobile through multiplelinear regression and binomial fitting each level of independent variables. Results： The optimum extraction conditions were as follows： the extraction temperature was 65℃, the ratio of material to liquid was 1∶ 45, thee xtraction time was 72 min, with twice extraction. The prediction value of the extraction rate was 7. 49%, andthe actual average extraction rate was 7. 57% with relative error of 0. 8%. Conclusion： The central composite design - response surface methodology is simple and reasonable with high stability and high accuracy to optimize polysaccharide extraction from Dendrobium nobile, and the predictability is better.

关 键 词：金钗石斛 多糖 星点设计-响应面法 提取工艺 

分 类 号：R284[医药卫生—中药学]