南方红豆杉枝叶紫杉烷类萃余物中黄酮苷类成分研究  

Flavonoid Glycosides from Taxus chinensis var. Mairei Remainder Extracts Free of Taxoids

作　　者：李兆慧[1] 高兴兴 喻晓雁[1] 阮晓[2] 王强[2] LI Zhao - hui;GAO Xing - xing;YU Xiao - yan;RUAN Xiao;WANG Qiang(College of Traditional Chinese Medicines,Zhejiang Pharmaceutical College,Ningbo315100,China;Department of Biological and Chemical Engineering,Ningbo Instituteof Technology,Zhejiang University,Ningbo 315100,China)

机构地区：[1]浙江医药高等专科学校中药学院,浙江宁波315100 [2]浙江大学宁波理工学院生化分院,浙江宁波315100

出　　处：《中医药信息》2018年第3期68-70,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.31670631);宁波市自然基金项目(No.2017A610263);浙江省教育厅师资队伍建设课题(No.HR2017003);浙江医药高等专科学校2017年度校级重点课题(No.ZPCSR2017002)

摘　　要：目的:研究南方红豆杉枝叶紫杉烷类萃余物(TREFT)的黄酮苷类成分。方法:TREFT粉末的紫杉黄酮粗提物采用AB-8大孔树脂、硅胶柱色谱,减压ODS柱、重结晶等方法进行分离纯化,通过MS、~1H-NMR、^(13)C-NMR等技术鉴定化合物结构。结果:从TREFT中分离得到3个化合物,分别鉴定为山奈酚3-O-β-D-鼠李糖(1″′→6″)-β-D-葡萄糖苷(1)、槲皮素3-O-芸香糖苷(2)、山奈酚3-O-α-L-阿拉伯糖(1″′→6″)-β-D-葡萄糖苷(3)。结论:化合物1和3首次从红豆杉属植物中分离得到。Objective： To investigate the flavonoid glycosides from Taxus chinensis var. Mairei remainder extracts free of taxoids （ TREFT）. Methods： The flavonoids glycosides were isolated and purified by AB - 8 macroporous resin, silica gel chromatography, ODS and recrystalllzation. The structures were elucidated by spectroscopic analyses and comparison of MS, 1H - NMR, 13C - NMR data with those reported in literature.Results： Three flavonoid glycosides were identified as 3 - O - β - D - rhamnopyranosyl （1″′ →6″） - β - D -glucopyranoside （1 ）, quercetin 3 - O - rutinoside （2 ）, kaempferol 3 - O - α - L - arabinopyranosyl（1″′ →6″） - β - D - glucopyranoside （3）. Conclusion： Compounds 1 and 3 were obtained from this genus forthe first time.

关 键 词：TREFT 黄酮苷 山柰酚3-O-β-D-鼠李糖(1'''→6'')-β-D-葡萄糖苷 槲皮素3-O-芸香糖苷 山柰酚3-O-α-L-阿拉伯糖(1'''→6'')-β-D-葡萄糖苷 

分 类 号：R284[医药卫生—中药学]