HPLC法同时测定闽产圆齿野鸦椿中两种色原酮碳苷的含量  

Simultaneous Determination of Isobiflorin and Biflorin in Fujian Euscaphis japonica by HPLC

作　　者：倪林[1,2] 邱亚铁 李艳蕾 邹小兴 徐会有 NI Lin1,2, QIU Yatie1, LI Yanlei2,3, ZOU Xiaoxing2,3, XU Huiyou1(1.College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China ; 2.Fujian Colleges and Universities Engineering Research Institute of Conservation ＆ Utilization of Natural Bioresources, Fujian Agriculture and Forestl3~ University, Fuzhou 350002, China; 3.College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Chin)

机构地区：[1]福建农林大学植物保护学院,福建福州350002 [2]自然生物资源保育利用福建省高校工程研究中心,福建福州350002 [3]福建农林大学林学院,福建福州350002

出　　处：《林产化学与工业》2018年第4期103-108,共6页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(31700292);福建农林大学科技创新专项基金资助(CXZX2016073)

摘　　要：建立了HPLC同时测定圆齿野鸦椿中两个成分5,7-二羟基-2-甲基-色原酮-8-C-β-D-葡萄糖苷(A)和5,7-二羟基-2-甲基-色原酮-6-C-β-D-葡萄糖苷(B)的方法,并测定了这两个成分在闽产圆齿野鸦椿枝条、叶片、果皮和种子中的含量。结果表明:HPLC测试条件为色谱柱DikmaDiamonsil C18柱(250 mm×4.6 mm,5μm),流动相甲醇/水(25∶75,体积比),等度洗脱,流速为1.0 mL/min;检测波长256 nm,柱温30℃,检测时间15 min时,化合物A和B分别在2.000 2～20.002 0μg和2.001 6～20.016 0μg范围内线性关系良好(r=0.999 9),平均加样回收率分别为98.42%和99.77%,RSD均小于1.00%(n=6)。HPLC法快速简便、重复性和稳定性好、结果准确可靠,可为今后圆齿野鸦椿在药用植物资源开发应用方面提供依据。The method for simultaneous determination of isobiflorin and biflorin in Euscaphis japonica （Thunb.） Dippel was established by HPLC, and their contents in twigs, leaves, epicarp, and seeds of E. japonica from 10 different producing areas in Fujian province were determined. The determination was carried out on Dikma Diamonsil C 18 column （250 mm×4.6 mm, 5 μm） with anisocraticmobile phaseof 25% methanol.The flow rate was 1.0 mL/min, the UV detection wavelength was set at 256 nm, the column temperature was 30 ℃ , and the detection time was 15 min. The isobiflorin（ A ） and biflorin（ B ） showed good linearity in the range of 2.000 2-20.002 0 μg （ r =0.999 9） and 2.001 6-20.016 0 μg （ r =0.999 9）, respectively. The average recoveries of isobiflorin and biflorin were 98.42% and 99.77% with the RSD of 0.88% and 0.96%, respectively. The developed method was found to be rapid, stable and accurate, and could be used to control the quality of E. japonica development and application of traditional Chinese medicine.

关 键 词：野鸭椿属 色原酮碳苷 高效液相色谱法 

分 类 号：TQ35[化学工程] TQ91