肝窦内皮细胞对肝细胞生物学特性影响的体外研究  

Function of liver sinusoidal endothelial cells in the biological characteristics of liver cells in vitro

作　　者：符思远 罗杏英[2] 门秀丽 张文健[4] 娄晋宁 许诚[6] 李宓[2] FU Siyuan;LUO Xingying;MEN Xiuli;ZHANG Wenjian;LOU Jinning;XU Cheng;LI Mi(Department of Nephrology,Dongguan Tung Wah Hospital,Dongguan 523000,China;Blood Puriftcation Center,the Fifth Affiliated Hospital of Sun Yat- sen University,Zhuhai 519000,China;Department of Pathophysiology,North China U）tiversity of Science and Technology,Tangshan 063009,China;Institute of Clinical Medicine,China-Japan Friendship Hospital,Beijing 100029,China;Suzhou Rui Lai Biological Technology Corporation,Suzhou 215000,China;Department of Liver Disease,the Third People＇s Hospital of Shenzhen City,Shenzhen 518000,China)

机构地区：[1]东莞东华医院肾内科,广东东莞523000 [2]中山大学附属第五医院血液净化中心,广东珠海519000 [3]华北理工大学基础学院病理生理系,河北唐山063009 [4]中日友好医院临床医学研究所,北京100029 [5]苏州瑞徕生物科技有限公司,江苏苏州215000 [6]深圳市第三人民医院肝病医学部,广东深圳518000

出　　处：《东南大学学报（医学版）》2018年第4期566-572,共7页Journal of Southeast University(Medical Science Edition)

基　　金：国家高技术发展研究计划("863"计划)项目(2006AA02Z482)

摘　　要：目的:探讨永生化人肝窦内皮细胞(IHLESC)能否提高肝细胞的代谢、解毒及合成功能。方法:设立永生化人肝细胞(IHH)单独培养组和IHLESC与IHH共培养组,评价两种不同培养方式对肝细胞代谢、解毒和合成功能的影响;将两组细胞分别接种于微载体上,分别与肝衰竭患者血浆共孵育,比较两组IHH的解毒功能。结果:相比于单独培养组,共培养组IHH密切接触,聚集成团。共培养组IHH的谷氨酰胺合成酶(GS)、谷胱甘肽-S-转移酶(GST)、鸟苷二磷酸葡萄糖醛酸基转移酶1A1(UGT1A1)、白蛋白(ALB)表达量均显著高于单独培养组(P<0.01)。共培养组血浆总胆红素的下降及血浆尿素的上升均比单独培养组显著(P<0.01)。结论:用IHH与IHLSEC在微载体上共培养制备成新型生物人工肝的核心材料,可提高生物人工肝细胞的代谢、解毒及合成功能。Objective： To investigate whether human liver sinusoidal endothelial cells can improve metabolism,detoxification and synthesis via co-culture of immortalized human hepatocytes（ IHH） with immortalized human liver sinusoidal endothelial cells（ IHLSEC）. Methods： Two experimental groups were set up： IHH single culture,and IHLSEC and IHH co-culture groups. Metabolism,detoxification and synthesis in both cell groups were compared.Sequentially,the two groups cultured in micro-carriers were respectively incubated with plasma from patients with liver failure to compare detoxification function between the two groups. Results： Compared to the single culture group,IHH in the co-culture group had tight contact between cells,which were clumped together. The expression of GS,GST,UGT1 A1,and ALB in the co-culture group was significantly higher than the other（ P〈0. 01）. Coculture group decreased plasma urea and increased plasma total bilirubin more significantly than the single group（ P〈0. 01）. Conclusion： The new bioartificial liver,constituted of IHLSEC and IHH attached to micro-carriers,may have better metabolism,detoxification,synthesis,and functionality.

关 键 词：永生化人肝细胞 永生化人肝窦内皮细胞 共培养 肝功能衰竭 

分 类 号：R318.14[医药卫生—生物医学工程]