灰树花提取成分对小鼠免疫功能影响的实验研究  

Effects of Grifola frondosa extracts on mouse immunity

作　　者：温斌[1] 吕昌龙[1] 曹雅明[1] 佐佐木甚一[2] 刘军[1] 阎建忠[1] 

机构地区：[1]中国医科大学免疫学教研室,沈阳110001 [2]日本弘前大学医学部

出　　处：《中国免疫学杂志》2002年第8期550-552,共3页Chinese Journal of Immunology

摘　　要：目的:以NK细胞杀伤活性,巨噬细胞吞噬功能及TNFα,IL-1的产生水平为指标,对灰树花乙醇沉淀物（ET-Pre）和RNA提取物影响小鼠非特异性免疫功能及其抗肿瘤机制作初步探讨。方法:以常规生物学测定的乳酸脱氢酶法,吞噬中性红法,结晶紫染色法,MTT生物活性测定法进行检测。结果:灰树花ET-Pre和RNA提取物处理组小鼠脾NK细胞杀伤活性,巨噬细胞吞噬功能及TNF-α,IL-1的产生水平均较对照组呈现有显著意义的增高或增强。并且灰树花RNA提取物提高NK细胞杀伤活性和巨噬细胞吞噬功能的程度大于灰树花ET-Pre的作用。结论:灰树花两种提取物可增强机体非特异性免疫功能,间接发挥抗肿瘤作用。Objective :To investigate the effects of ethanol precipitate(ET-Pre) and RNA of Grifola frondosa on non-specific immunity in mouse.Methods:The common biological methods were used to examine the levels of cytokines and immunocyte activity. Results: The killing activity of the NK cells, the phagocytosis function of macrophages and the levels of TNFα/IL-1 in the animals treated with ET-Pre and RNA respectively were significantly higher than those in the control.The RNA was stronger than ET-Pre in increasing killing activity of NK cells and phagocytosis function of macrophages. Conclusion:Both ET-Pre and RNA extracts of Grifola frondosa may promote immunoactivity nonspecifi-cally and inhibit tumor cells indirectly.

关 键 词：灰树花提取成分 小鼠 免疫功能 实验研究 自然杀伤细胞 巨噬细胞 肿瘤坏死因子Α 白细胞介素1 

分 类 号：R285.5[医药卫生—中药学] R392.11[医药卫生—中医学]