丹参酮ⅡA磺酸钠联合顺铂对小鼠lewis肺癌细胞上皮性钙黏蛋白表达的影响  

Effect of STS and DDP on expression of E-cadherin in mouse lewis lung cancer cells

作　　者：王琳[1] 胡凯文[1] 迟佳琪 迟笑怡[1] 周天[1] WANG Lin, HU Kaiwen, CHI Jiaqi, et al.(Department of Oncology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, Chin)

机构地区：[1]北京中医药大学东方医院肿瘤科,硕士研究生100078

出　　处：《环球中医药》2018年第7期1000-1004,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81403250)

摘　　要：目的探讨活血化瘀药丹参酮ⅡA磺酸钠(STS)联合顺铂(DDP)对小鼠lewis肺癌细胞上皮性钙黏蛋白(E-cadherin)表达的影响。方法运用CCK8法摸索STS不同浓度对肿瘤细胞的抑制率影响;Western-blot检测E-cadherin蛋白的表达水平;Transwell小室侵袭转移实验检测各组细胞转移数量。结果浓度为0.1 mg/mL、0.2 mg/mL、0.4 mg/mL的STS与空白对照组比较,细胞的抑制率明显升高且呈浓度依赖性(P<0.05);STS联合DDP处理后,肺癌细胞侵袭能力和黏附能力明显减弱(P<0.05);药物处理后E-cadherin蛋白表达水平明显上升(P<0.05)。结论活血化瘀药STS联合DDP能调节小鼠lewis肺癌细胞E-cadherin表达并抑制其侵袭转移,在肺癌转移过程中具有重要作用。Objective To explore the effects of STS for invigorating blood circulation and eliminating stasis on the E-cadherin expression of mouse lewis lung cancer cell. Methods Effect of STS on GFPLLC inhibition ratio was measured by cell hyperplasia by CCK8 assay. Effect of altered drug concentration of STS on cell migration was measured by cell invasion by Transwell chamber and cell adhesion by adhesion assay respectively. Expression levels of E-cadherin protein were assayed by Western blot. Results When the concentration of 0. 1 mg/mL,0. 2 mg/mL and 0. 4 mg/mL STS,compared with the blank control group,the inhibition rate of the cells was obviously increased,and it was concentration dependent（ P〈0.05）. STS and DDP treated lung cancer cell invasion and adhesion ability decreased significantly（ P〈0.05）; the expression level of E-cadherin protein increased significantly（ P〈0. 05） with the control cells.Conclusion The migration,invasion and adhesion of mouse lewis lung cancer cell are regulated by the combination of STS and DDP. The combination of STS and DDP may play an important role in the invasion and metastasis of lung cancer.

关 键 词：小鼠LEWIS肺癌细胞 丹参酮ⅡA磺酸钠 顺铂 肿瘤细胞侵袭 细胞黏附 上皮性钙黏蛋白 

分 类 号：R285.5[医药卫生—中药学]