芒果苷对脊髓损伤模型中氧化应激反应及线粒体途径凋亡的调节作用  

The regulating effects of mangiferin on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model

作　　者：李前凯[1] 李江梅 陈石岩[2] LI Qian-kai;LI Jiang-mei;CHEN Shi-yan(Rehabilitation Medicine Department,the First People＇s Hospital of Kashgar Prefecture in Xinjiang,Kashgar,Xinjiang,844000,China;Department of Pathology,the First People＇s Hospital of Kashgar Prefecture in Xinjiang,Kashgar,Xinjiang,844000,China)

机构地区：[1]新疆喀什地区第一人民医院康复医学科,新疆喀什844000 [2]新疆喀什地区第一人民医院病理科,新疆喀什844000

出　　处：《海南医学院学报》2018年第10期979-981,985,共4页Journal of Hainan Medical University

基　　金：喀什地区科技计划项目(ks2017039)~~

摘　　要：目的:研究芒果苷对脊髓损伤模型中氧化应激反应及线粒体途径凋亡的调节作用。方法:选择SD大鼠作为实验动物并分为进行假手术操作的对照组、制作脊髓损伤模型的损伤组、制作脊髓损伤模型并给予芒果苷干预的芒果苷组。干预后30天,取脊髓组织并采用放射免疫沉淀试剂盒测定氧化应激指标ROS、H_2O_2、SOD、GSH-Px、CAT的含量,采用酶联免疫吸附试剂盒测定细胞凋亡指标SKIP、ASK1、Fas、tBid、APAF-1、Caspase-3的含量。结果:损伤组大鼠脊髓组织中ROS、H_2O_2、SKIP、ASK1、Fas、tBid、APAF-1、Caspase-3的含量显著高于对照组,SOD、GSH-Px、CAT的含量显著低于对照组;芒果苷组大鼠脊髓组织中ROS、H_2O_2、SKIP、ASK1、Fas、tBid、APAF-1、Caspase-3的含量显著低于损伤组,SOD、GSH-Px、CAT的含量显著高于损伤组。结论:芒果苷能够对脊髓损伤模型中氧化应激反应及线粒体途径凋亡具有抑制作用。Objective：To study the regulating effects of mangiferin on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model.Methods：SD rats were selected as the experimental animals and divided into the control group that underwent sham operation,the injury group that were made into spinal cord injury models and the mangiferin group that were made into spinal cord injury models and given mangiferin intervention.30 days after the intervention,spinal cord tissue was collected,radioimmunoprecipitation kit was used to determine the contents of oxidative stress indexes ROS,H_2O_2,SOD,GSH-Px and CAT,and enzyme-linked immunosorbent assay kit was used to determine the contents of apoptosis indexes SKIP,ASK1,Fas,tBid,APAF-1 and Caspase-3.Results：ROS,H_2O_2,SKIP,ASK1,Fas,tBid,APAF-1 and caspase-3 contents in spinal cord tissue of injury group were significantly higher than those of control group whereas SOD,GSH-Px and CAT contents were significantly lower than those of control group;ROS,H_2O_2,SKIP,ASK1,Fas,tBid,APAF-1 and caspase-3 contents in spinal cord tissue of mangiferin group were significantly lower than those of injury group whereas SOD,GSH-Px and CAT contents were significantly higher than those of injury group.Conclusion：Mangiferin has inhibitory effect on oxidative stress response and mitochondrial pathway apoptosis in spinal cord injury model.

关 键 词：脊髓损伤 芒果苷 氧化应激反应 细胞凋亡 

分 类 号：R681[医药卫生—骨科学]