CO_2浓度对大豆叶片气孔特征和气体交换参数的影响  

Effects of CO_2 Concentrations on Stomatal Traits and Gas Exchange in Leaves of Soybean

作　　者：李菲[1] 刘亮[1] 张浩 王清涛 郭丽丽[1] 郝立华[1] 张茜茜 曹旭 梁伟佳 郑云普[1] LI Fei;LIU Liang;ZHANG Hao;WANG Qing-Tao;GUO Li-Li;HAO Li-Hua;ZHANG Xi-Xi;CAO Xu;LIANG Wei-Jia;and ZHENG Yun-Pu(School of Water Conservancy and Hydroelectric Power,Hebei University of Engineering,Handan 056038,Hebei,Chin;School of Life Science and Engineering,Handan University,Handan 056005,Hebei,China;School of Landscape and Ecological Engineering,Hebei University of Engi-neering,Handan 056038,Hebei,China)

机构地区：[1]河北工程大学水利水电学院,河北邯郸056038 [2]邯郸学院生命科学与工程学院,河北邯郸056005 [3]河北工程大学园林与生态工程学院,河北邯郸056038

出　　处：《作物学报》2018年第8期1212-1220,共9页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31400418);河北省自然科学基金项目(C2016402088;E2016402098);河北省创新能力提升计划科技研发平台建设专项"河北省水资源高效利用工程技术研究中心"(18965307H);河北省高等学校青年拔尖人才计划项目(BJ2016012);河北省引进留学人员资助项目(CN201702);中国博士后科学基金项目(2014M561044;2016T90128);河北省教育厅青年科学基金项目(QN2015253)资助~~

摘　　要：利用可精准控制CO_2浓度的大型气候箱设置7个CO_2浓度处理(400、600、800、1000、1200、1400和1600μmol mol^(–1)),对大豆进行CO_2浓度富集的室内培养试验。结果表明,CO_2浓度升高显著减小大豆叶片近轴面的气孔密度和远/近轴面的气孔面积指数。当CO_2浓度为400μmol mol^(–1)时,远轴面气孔分布最规则,提高CO_2浓度导致远轴面气孔的不规则分布;与远轴面相反,CO_2浓度升高导致近轴面气孔的空间分布更加规则,即在较高CO_2浓度处理下的Lhat(d)最小值均低于对照组。不同叶面(远/近轴面)气孔特征对大气CO_2浓度变化的响应存在明显差异,但大豆可以通过调整气孔形态特征和气孔空间分布格局进一步改变叶片的气体交换参数。研究结果有助于从气孔特征响应的角度深入理解CO_2浓度对大豆叶片气体交换过程产生的影响。Seven concentrations treatments （400, 600, 800, 1000, 1200, 1400, and 1600 μmol mol-1） were designed to investigate the effects of atmospheric CO2 concentrations on the stomatal traits and leaf gas exchange of soybean. We found that elevating CO2 concentrations significantly decreased the stomatal density of adaxial side and the stomatal area index of both the adaxial and abaxial sides. Meanwhile, the spatial distribution pattern analysis of stomata with the Ripley＇s K function showed that the spatial distribution pattern of stomata on leaf surfaces of soybean was highly scale-dependent. The most regular distribution pattern of stomata on the abaxial surface was found under the CO2 concentration of 400 μmol mol-1, and the increase of CO2 concentration resulted in irregular distribution pattern of stomata on the abaxial surface of soybean leaves. In contrast to the abaxial surface, elevating CO2 concentrations made the spatial distribution pattern of stomata more regular on the adaxial leaf surface, which wasevidenced by lower minimal Lhat（d） values under elevated CO2 concentrations than those under CO2 concentration of 400 μmol mol–1. Although the response of stomatal traits to atmospheric CO2 concentration was obviously different between the adaxial and abaxial surfaces of leaves, soybean plants could alter leaf gas exchange through adjusting the morphological traits and the spatial distribution pattern of stomata. These results may be helpful for further understanding potential mechanisms concerning about the elevating CO effect on the leaf gas exchange of soybean plants from the view of stomatal traits.

关 键 词：CO2 浓度 大豆 气孔结构特征 气孔空间分布 气体交换参数 

分 类 号：S565.1[农业科学—作物学]