氯化十六烷基吡啶-肝素体系的共振瑞利散射光谱及其应用  

Resonance rayleigh scattering of cetylpyridinium chloride-heparin system and its application

作　　者：霍燕燕[1] 韩权[1] 李欣 田丽[2] 杨晓慧[1] HUO Yanyan;HAN Quan;LI XIN;TIAN Li;YANG Xiaohui(School of Chemical Engineering,Xi＇an University,Xi＇an,Shaanxi,710065 P.R.China;Center of Physical Tesing and Chemical Analysis Center,Shaanxi Provieial Disease Control and Prevention Center,Xi＇an,Shaanxi,710045 P.R.China)

机构地区：[1]西安文理学院化学工程学院,陕西西安710065 [2]陕西省疾病预防控制中心,陕西西安710054

出　　处：《华西药学杂志》2018年第4期395-397,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:21545014);西安市科技计划项目(2016CXWL09);陕西省教育厅项目(17JK1127);陕西省省级重大创新项目(201736)

摘　　要：目的基于肝素（Hep）对氯代十六烷基吡啶（CPCM）共振瑞利散射的增强作用，建立新的测定肝素的方法。方法在pH4．5～6．5缓冲溶液中，Hep—CPCM体系有一灵敏的散射光谱，最大散射峰在293nm处，在542nm处有一个较弱的吸收峰。结果0．04～1．2mg·L^-1Hep与散射强度符合线性关系，方法的检出限为0．011mg·L^-1。结论所用方法简便、检出限低，可用于注射液中Hep的测定。OBJECTIVE To develop a novel method for the determination of heparin based on the enhancement of Resonance rayleigh scattering （RRS） of cetylpyridinium chloride in the presence of heparin. METHODS In pH4.5 - 6.5 britton - robinson buffer solution ,the RRS signal of heparin and cetylpyridinium chloride alone was very weak,but it was etahaneed greatly and a new RRS spectra appeared when they interacted with each other to form the aion - association complex. The strong peak was at 293 nm and the weak peak was at 542 nm. RESULTS The mass concentration of heparin in the range of 0.04 - 1.2 mg· L^- 1 was linear to the intensity of RRS. The detection limit was 0.011mg· L^- 1 CONCLUSION The method is simple, it has low detection limit and can determine the heparin sodium in injections with satisfactory results.

关 键 词：肝素 氯化十六烷基吡啶 注射液 共振瑞利散射 测定 肝素钠 荧光 葡糖胺聚糖 

分 类 号：R917[医药卫生—药物分析学]