两株链霉菌对小麦幼苗生长及诱导抗性的影响  

Effects of Two StreptomycesStrains on Growth and Induced Resistance of Wheat Seedlings

作　　者：刘玉涛[1] 张凯 马军妮 来航线[1] 薛泉宏[1] LIU Yutao;ZHANG Kai;MA Junni;LAI Hangxian;XUE Quanhong(College of Resources and Environment,Northwest A~-F University,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《西北农业学报》2018年第5期658-666,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十二五"国家科技支撑计划(2012BAD14B11);陕西省科技统筹创新工程计划(2016KTZDNY03-03-02)~~

摘　　要：研究2株链霉菌D74和Act12对小麦生长的促进作用及诱导抗性的影响,为链霉菌剂在小麦生产中的应用提供科学依据。通过皿内发芽试验、砂培和土培盆栽试验,测定2种链霉菌的混合制剂(1∶1质量比)在不同用量及处理方式下的小麦发芽率、发芽指数、种子活力、幼苗生物学性状、叶片诱导抗性及生化特性。结果表明:(1)供试链霉菌具有显著的促生功能。菌粉包衣量为每粒10mg时,小麦发芽率较对照提高41.9%(P<0.05),发芽指数提高40.0%(P<0.05);叶面积增加149.2%(P<0.05),植株总鲜质量增加100.0%(P<0.05),根系鲜质量增加220.0%(P<0.05)。10倍菌粉浸提稀释液浸种,种子活力较对照提高31.8%(P<0.05);包衣量每粒5mg处理根系活力提高62.7%(P<0.05);10倍发酵稀释液浸种,小麦株高、叶面积较对照分别增加29.2%、27.6%(P<0.05),稀释10~2~10~4倍发酵液浸种,小麦出苗率较对照提高44.0%~103.8%(P<0.05)。(2)供试链霉菌可提高小麦诱导抗性。适量菌粉种子包衣及发酵滤液浸种可使小麦叶片POD酶活性较对照提高46.7%~70.0%(P<0.05);菌粉包衣量每粒5mg处理小麦叶片PPO酶活性较对照提高52.5%(P<0.05),10~2~10~4倍发酵滤液浸种,小麦叶片MDA质量摩尔浓度降低34.0%~40.2%(P<0.05)。可见,供试链霉菌对小麦具有显著的促生作用,并能提高小麦的诱导抗性。In this study,the effects of two Streptomyces strains,S.roche D74 and S.partum Act12,on growth promotion and induced resistance in wheat were examined,in order to provide evidence for the application of streptomycete agents in wheat production.A mixed agent of the two test strains（1∶1 mass ratio）was applied at different rates and using different methods in petri dish germination,sand pot,and soil pot experiments.The germination rate,germination index,seed vigor,seedling growth parameters and induced resistance indices of wheat were analyzed.The results showed that（i）the mixed Streptomyces agent significantly promoted the growth of wheat.Compared with the control,the treatment of seed coating at 10 mg/per grain increased the germination rate by 41.9%,the germination index by 40.0%,the leaf area by 149.2%,the total fresh mass of the plant by 100%,and the fresh mass of roots by 220.0%（P〈0.05 for all）.The seed vigor increased by 31.8% in the treatment of seed soaking in 10-fold-diluted extract,while larger increase（62.7%）occurred in the root activity in the treatment of coating at 5 mg/per grain compared with the control（P〈0.05 for both）.The plant height and leaf area of wheat seedlings showed similar increases（29.2% and 27.6%,respectively）in the treatment of seed soaking in 10-fold-diluted fermentation filtrate,and the emergence rate of seedlings increased by 44.0%-103.8% in the treatment of seed soaking in the 10^-2-10^-4 dilutions of fermentation filtrate（P〈0.05 for all）.（ii）The mixed Streptomyces agent markedly improved induced resistance in wheat.Leaf POD activity increased by 46.7%-70.0% in the treatments of seed coating and soaking at appropriate rates,while 52.5%increase occurred in leaf PPO activity in the treatment of seed coating at 5 mg per grain compared with the control（P〈0.05 for both）.However,a decrease of 34.0%-40.2% was found in leaf MDA content in the treatment of seed soaking in the 10^-2-10^-4 dilutions of fermentation filtrate compared with the contr

关 键 词：链霉菌 促生作用 诱导抗性 小麦 

分 类 号：S182[农业科学—农业基础科学] Q939.9[生物学—微生物学]