青蒿素对老年小鼠学习记忆及炎性细胞因子和单胺类神经递质的影响  

Effects of artemisinin on learning and memory, inflammatory cytokines and monoamine neurotransmitters in aged mice

作　　者：王光辉[1] 钟鸣[1] 郑公朴 高慧婕[1] 高红刚[1] 吴娉 刘安信 吴景龙[3] Wang Guanghui;Zhong Ming;Zheng Gongpu;Gao Huijie;Gao Honggang;Wu Ping;Liu Anxin;Wu Jinglong(Department of Immune Pharmacology Teaching and Research,College of Pharmacy,Jining Medical University,Rizhao 276826,Chin;Department of Senile Diseases,Rizhao Hospital of Traditional Chinese Medicine,Rizhao 276826,China;Brain Science and Neurotechnology Lab,Beijing Institute of Technology,Beijing 100081,Chin)

机构地区：[1]济宁医学院药学院免疫药理学教研室,日照276826 [2]日照市中医医院老年病科,日照276826 [3]北京理工大学脑科学与神经技术实验室,北京100081

出　　处：《中华行为医学与脑科学杂志》2018年第7期593-597,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（61473043）;山东省重点研发计划（2018GSF119006）;济宁医学院青年科学基金项目（JY2015KJ005）;济宁市科技发展计划项目（2015-57-127）

摘　　要：目的探讨青蒿素对老年小鼠记忆能力的改善作用及对炎性细胞因子和单胺类神经递质的影响。方法30只22月龄老年小鼠随机分成老年小鼠模型对照组、青蒿素小剂量组（饲料中含青蒿素0．1％）和青蒿素大剂量组（饲料中含青蒿素0．3％），每组10只。另设10只2月龄小鼠作为青年小鼠模型对照组；给药组连续10周喂饲含青蒿素饲料，老年小鼠模型对照组和青年小鼠模型对照组喂饲标准饲料。采用Morris水迷宫实验检测小鼠的记忆能力、Elisa法检测小鼠血清炎性因子（IL-6和TNF-α）水平、HPLC法分析小鼠脑中神经递质（多巴胺、去甲肾上腺素和5-羟色胺）的含量。结果（1）水迷宫隐形平台实验中，老年小鼠模型对照组与青年小鼠模型对照组相比在水中滞留时间明显延长（P〈0．05）；青蒿素小剂量组和青蒿素大剂量组与老年小鼠模型对照组相比在水中滞留时间明显缩短（P〈0．05）。在反向隐性平台实验的第9天，青蒿素小剂量组[（50．1±19．9）S]及青蒿素大剂量组[（43．2±17．6）s]在水中滞留时间与老年对照组[（66．1±29．1）S]相比显著缩短（P〈0．05）。（2）血清炎性因子测定实验中，青蒿素小剂量组[IL-6：（28．4±4．3）pg／ml，TNF—α：（51．8±8．2）pg／ml]和青蒿素大剂量组[IL-6：（17．6±2．3）pg／ml，TNF-α：（38．6±12．5）pg／ml]IL-6与TNF—α水平与老年小鼠模型对照组[IL-6：（36．12±7．98）pg／ml），TNF-α：（67．32±10．27）pg／ml）]相比显著降低（P〈0．05）。（3）脑组织神经递质含量测定中，青蒿素小剂量组DA[（19．96±3．89）mmol／ml]、5-HT[（5．73±0．93）mmol／ml]含量高于老年小鼠模型对照组，青蒿素大剂量组DA[（26．13±5．66）mmol／ml]、NE[（16．31±2．69）mmol／ml]、5-HT[（8．03±1．93）mmol／ml]含量高于老年小鼠模型对照组[DA（13．96±3．89）mmol�Objective To investigate whether the arterrnisinin has beneficial efficacy to improve the learning and memory in aged mice, as well as the possible mechanisms regarding the inflammatory cytokines and monoamlne neurotransmitters. Methods 30 aged mice（22 month old） were randomly divided into the aged mouse model control group （n= 10）, the artemisinin low dose group （artemisinin 0. 1% in feed, n= 10 ） and the artemisinin high dose group （artemisinin 0.3% in feed, n= 10）. Another 10 mice （2 month old） served as young mouse model eontrol group. The artemisinin low dose group and the artemisinin high dose group fed artemisinin feed for 10 weeks. The aged mouse model control group and the young mouse model control group were fed standard feed.Morris water maze test was performed to assess learning and memory capacities for evaluation of the cognitive degree. Serum TNF-α and IL-6 were also detected by ELISA and dopamine, norepinephrine, serotonin in the brain were analyzed by HPLC.Results （ 1 ） Morris water maze test showed, the retention time of the aged mouse model control group was significantly longer than that of the young mouse model eontrol group （P〈0.05）. The retention time of the artemisinin low dose group and the artemisinin high dose group was significantly shorter than that of the aged mouse model control group （P〈 0.05）. In the ninth days of the reverse reeessive platform experiment, the retention time of the artemisinin low dose group （（50.1＋19.9） s） and the artemisinin high dose group （（43.2±17.6） s） was significantly shorter than that of the aged mouse model control group （（66.1±29.1） s ,P〈0.05）. （2） Serum inflammatory factors test showed, the level of IL-6 and TNF-α in the artemisinin low dose group （ IL-6 ： （ 28.4 ± 4.3） pg/ml,TNF-α： （51.8±8.2） pg/ml） and the artemisinin high dose group（ IL-6 ：（17.6±2.3） pg/ml, TNF-α ： （38.6±12.5） pg/ml） were significantly lower than those in aged mouse model co

关 键 词：青蒿素 老年小鼠 学习记忆 炎性因子 神经递质 

分 类 号：R285.5[医药卫生—中药学]