一测多评法测定不同味连饮片中6个生物碱类成分的含量  

Simultaneous determination of 6 alkaloids in prepared slides of Coptis chinensis Franch. by QAMS

作　　者：李听弦 李蒙 张志 谢婧 孔德暄 王光忠 刘艳菊 LI Ting-xian;LI Meng;ZHANG Zhi;XIE Jing;KONG De-xuan;WANG Guang-zhong;LIU Yan-ju(School of Pharmacy,Hubei College of Chinese Medicine,Wuhan 430065,China;Hubei Engineering Technology Research Center of Chinese Medicine Processing,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]湖北省中药炮制工程技术研究中心,武汉430065

出　　处：《药物分析杂志》2018年第7期1132-1138,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技基础性工作专项(2014FY111100-2)

摘　　要：目的:采用一测多评,建立味连饮片中非洲防己碱、药根碱、表小檗碱、黄连碱、巴马汀、小檗碱6个生物碱类成分的含量测定方法。方法:采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(含0.3%磷酸及0.2%三乙胺)为流动相,梯度洗脱,流速0.7 m L·min^(-1),柱温20℃,检测波长268 nm,建立小檗碱与非洲防己碱、表小檗碱、巴马汀、黄连碱、药根碱的相对校正因子,并以相对保留时间作为目标峰的定位标准。结果:色谱峰分离度良好,非洲防己碱、药根碱、表小檗碱、黄连碱、巴马汀、小檗碱进样量分别在0.118~1.18、0.176~1.76、0.190~1.90、0.272~2.72、0.198~1.98、0.98~9.80μg范围内呈良好线性关系,平均加样回收率分别为102.4%、98.1%、100.2%、102.6%、103.6%、103.3%。小檗碱对非洲防己碱、药根碱、表小檗碱、黄连碱、巴马汀的相对校正因子分别为1.351、1.094、1.304、1.361、1.451;12批味连饮片中6个生物碱类成分一测多评法计算值与实测值间均无显著性差异(RE<5%)。结论:采用一测多评法测定味连中生物碱类成分的含量准确、可行。Objective：To establish the content determination method of 6 alkaloids（columbamine,jatrorrhizine,epiberberine,coptisine,palmatine and berberine）in prepared slides of Coptis chinensis.Methods：C_（18）chromatographic column（250 mm×4.6 mm,5μm）was adopted,the mobile phase was acetonitrile-water（containing 0.3%phosphoric acid and triethylamine）at a flow rate of 0.7 m L·min^（-1）.The column temperature was 20℃and the detection wavelength was 268 nm.The relative correction factors of berberine to columbamine,jatrorrhizine,epiberberine,coptisine and palmatine were determined,and the relative retention time was used for peak identification.Results：The chromatographic peaks were separated well.The linear ranges of columbamine,jatrorrhizine,epiberberine,coptisine,palmatine and berberine were 0.118 to 1.18μg,0.176 to 1.76μg,0.190to 1.90μg,0.272 to 2.72μg,0.198 to 1.98μg,and 0.98 to 9.80μg,respectively.The average recoveries were102.4%,98.1%,100.2%,102.6%,103.6%and 103.3%,respectively.The relative correction factors of berberine to columbamine,jatrorrhizine,epiberberine,coptisine,and palmatine were 1.351,1.094,1.304,1.361 and 1.451,respectively.There was no significant difference（RE5%）between the calculated values and the measured values of 6 alkaloids in 12 batches of prepared slides of Coptis chinensis Franch.Conclusion：The proposed QAMS method for determinantion of alkaloids in Coptis chinensis Franch.is accurate and feasible.

关 键 词：一测多评 校正因子 黄连 味连 生物碱 非洲防己碱 药根碱 表小檗碱 黄连碱 巴马汀 小檗碱 

分 类 号：R917[医药卫生—药物分析学]