超高效液相色谱法同时测定三七的叶和花中3个皂苷类成分含量  

Simultaneous determination of three saponins in leaves and flower buds of Panax notoginseng by UPLC

作　　者：雷蓉 赵振霞 刘永利 冯丽 魏锋[2] LEI Rong;ZHAO Zhen-xia;LIU Yong-li;FENG Li;WEI Feng(Hebei Institute for Drug Control,Shijiazhuang 050011,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]河北省药品检验研究院,石家庄050011 [2]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2018年第7期1146-1151,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：国家"重大新药创制"科技重大专项(2014ZX09304307-002)

摘　　要：目的:建立超高效液相色谱法测定三七的叶和花中人参皂苷Rb_1、Rb_2和Rb_3的含量。方法:采用Waters ACQUITY BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),以乙腈-水为流动相进行梯度洗脱,流速0.4 m L·min^(-1),柱温30℃,检测波长203 nm。结果:人参皂苷Rb_1、Rb_2和Rb_3线性关系良好;三七叶中上述3个成分平均加样回收率(n=9)均为100.5%,三七花中上述3个成分平均加样回收率(n=9)分别为99.3%、99.7%、98.6%。12批三七叶样品中人参皂苷Rb_1、Rb_2和Rb_3含量范围分别为0.35%~0.59%、0.16%~0.68%、0.73%~3.33%,11批三七花样品中上述3个成分含量范围分别为0.46%~2.91%、0.83%~2.36%、2.95%~8.37%。结论:建立的方法可用于三七叶和三七花的含量测定。Objective：To establish a method for determination of ginsenoside Rb_1,ginsenoside Rb_2 and ginsenoside Rb_3 in leaves and flower buds of Panax notoginseng by UPLC.Methods：The analysis was performed on a Waters ACQUITY BEH C_（18） column（2.1 mm×100 mm,1.7μm）,with acetonitrile（A）-water（B）as the mobile phase with gradient elution at a flow rate of 0.4 m L·min^（-1）.The column temperature was 30℃and the detection wavelength was 203 nm.Results：The linear relationships of ginsenoside Rb_1,ginsenoside Rb_（2 ）and ginsenoside Rb_3 were favorable;the recoveries（n=9）of the above-mentioned components in leaves of Panax notoginseng were 100.5%,100.5%and 100.5%,respectively and the recoveries（n=9）of the above-mentioned components in flower buds of Panax notoginseng were 99.3%,99.7%and 98.6%%,respectively.The contents of ginsenoside Rb_1,ginsenoside Rb_2 and ginsenoside Rb_3 in 12 batches of leaves of Panax notoginseng were 0.35%-0.59%,0.16%-0.68%and 0.73%-3.33%,respectively.The contents of the three components in 11 batches of flower buds of Panax notoginseng samples were 0.46%-2.91%,0.83%-2.36%and 2.95%-8.37%,respectively.Conclusion：The established method can be used for determination of leaves and flower buds of Panax notoginseng.Keywords：leaves and flower buds of Panax notoginsengginsenosideprotopanoxadiol saponinsUPLC

关 键 词：三七叶、花 人参皂苷 原人参二醇型皂苷 超高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]