应用超声心动图评价PM_(2.5)暴露对小鼠左心室形态和功能的影响  

Echocardiographic evaluation of effect of PM_(2.5) exposure on left ventricular morphology and function in mice of different sexes

作　　者：曾雪娇[1] 杜喜浩 张佳[1] 潘坤 解玉泉[2] 蒋蓉芳[1] 赵金镯[1] 宋伟民[1] ZENG Xue-jiao;DU Xi-hao;ZHANG Jia;PAN Kun;XIE Yu-quan;JIANG Rong- fang;ZHAO Jin-zhuo;SONG Wei-min(Department of Environmental Health,School of Public Health,Fudan University,Shanghai 200032,China;Department of Cardiology,Xinhua Hospital,Shanghai Jiao Tong University,Shanghai 200092,China)

机构地区：[1]复旦大学公共卫生学院环境卫生学教研室,上海200032 [2]上海交通大学附属新华医院心内科,上海200092

出　　处：《环境与职业医学》2018年第7期596-601,共6页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:91543119;81673125)

摘　　要：[目的]探讨细颗粒物(PM_(2.5))的长期暴露对不同性别C57BL/6小鼠左心室形态和功能影响的差异。[方法]雌性和雄性C57BL/6小鼠各16只,随机分为PM_(2.5)过滤空气组(对照)和PM_(2.5)浓缩空气组(暴露),每组8只。利用"气象-环境动物暴露系统"进行PM_(2.5)暴露,每天8h,一周6d,共16周。暴露结束后,异氟醚吸入麻醉小鼠,高分辨率小动物超声影像系统测定小鼠左心室收缩和舒张末期内径、左心室收缩和舒张末期容积、左心室收缩和舒张末期室间隔厚度、收缩末期和舒张末期左心室后壁厚度、左心室质量等形态学指标,以及射血分数、左心室短轴缩短率、每搏输出量、心输出量、心率和RR间期等功能性指标。[结果]本次研究暴露仓内PM_(2.5)的平均质量浓度为(152.12±68.89)μg/m3,对照仓内为(9.45±0.99)μg/m3。与对照组相比,暴露组雌性小鼠左心室舒张末期内径[(3.03±0.34)mm]、左心室收缩末期内径[(1.82±0.21)mm]、左心室舒张末期容积[(36.51±9.98)μL]、左心室收缩末期室间隔厚度[(0.93±0.23)mm]、每搏输出量[(26.23±7.58)μL]、左心室质量[(54.85±15.35)mg]、心输出量[(13.47±4.16)mL/min]的水平均降低(P<0.05),对照组中各指标依次为:(3.69±0.37)mm、(2.47±0.37)mm、(58.41±14.16)μL、(1.21±0.15)mm、(36.08±7.64)μL、(80.52±20.82)mg、(21.05±7.41)μL;雄性小鼠PM_(2.5)暴露后,左心室形态和功能未出现明显变化。当小鼠均暴露于过滤空气环境中时,除了心率和舒张末期左心室后壁厚度外,雌性小鼠和雄性小鼠各指标差异无统计学意义;当小鼠均暴露于浓缩空气环境中时,雌性小鼠的左心室收缩末期内径、左心室舒张末期内径、左心室收缩末期容积、左心室舒张末期容积、舒张末期室间隔厚度、左心室质量、每搏输出量、心输出量均低于雄性小鼠(P<0.05)。[结论]PM_(2.5)暴露是影响左心室形态和功能的一个潜在危险因素,长期暴露对雌�[Objective] To evaluate the effect of long-term fine particulate matter （PM2.5） exposure on left ventricular morphology and function in C57BL/6 mice of different genders.[Methods] Sixteen female and sixteen male C57BL/6 mice were randomly divided into PM2.5 filtered air （control） group and PM2.5 concentrated air （exposure） group, with eight mice in each group. PM2.5 exposure was performed using a Meteorological and Environmental Animal Exposure System, eight hours daily, six days a week, for 16 weeks. After exposure, the mice were anesthetized by isoflurane inhalation to measure morphological indicators such as left ventricular end-systolic and end-diastolic diameters, left ventricular end-systolic and end-diastolic volumes, left ventricular end-systolic and end-diastolic interventricular septum thickness, left ventricular end-systolic and end-diastolic posterior wall thickness, and left ventricle mass, as well as functional indicators such as ejection fraction, left ventricular short axis shortening, stroke volume, cardiac output, heart rate, and RR interval by highresolution small animal ultrasound imaging system.[Results] The average concentration of PM2.5 in the exposure warehouse was （152.12±68.89） μg/m^3, and that in the control warehouse was （9.45±0.99） μg/m^3. Compared with the female mice in the control group [（3.69±0.37） mm, （2.47±0.37） mm, （58.41±14.16） μL, （1.21±0.15） mm, （36.08±7.64） μL, （80.52±20.82） mg, and （21.05±7.41） μL, respectively], the left ventricular enddiastolic diameter [（3.03±0.34） mm], left ventricular end-systolic diameter [（1.82±0.21） mm], left ventricle end-diastolic volume [（36.51±9.98） μL], left ventricular end-systolic septal thickness [（0.93±0.23） mm], stroke volume [（26.23±7.58） μL], left ventricle mass [（54.85±15.35） mg], and cardiac output [（13.47±4.16） mL/min] were reduced in the female mice in the exposure group （P 〈 0.05）; there was no significant change in the left ven

关 键 词：细颗粒物 超声心动图 小鼠 性别 左心室 

分 类 号：R122[医药卫生—环境卫生学]