大气细颗粒物对SH-SY5Y细胞能量代谢的影响  

Effect of airborne fine particulate matters on energy metabolism of SH-SY5Y cells

作　　者：班红芳 成丽霞 吕懿[1] 王丹 曹彬 穆箭兵 郑金平[1] BAN Hong-fang;CHENG Li-xial;LU Yi;WANG Dan;CAO Bin;MU Jian-bing;ZHENG JIN-ping(Department of Toxicology,School of Public Health,Shanxi Medical University,Taiyuan,Shanxi 030001,China;National Institutes of Health,Maryland 20899,USA)

机构地区：[1]山西医科大学公共卫生学院卫生毒理学教研室,山西太原030001 [2]美国国立卫生研究院,美国马里兰州20899

出　　处：《环境与职业医学》2018年第7期607-612,共6页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:30872137);山西省优势和特色重点学科建设项目(编号:无)

摘　　要：[目的]研究大气细颗粒物(PM_(2.5))对人神经母细胞瘤(SH-SY5Y)细胞能量代谢的影响。[方法]用不同质量浓度(下称浓度)PM2.50、20、40、80、160、320mg/L)染毒SH-SY5Y细胞24h,MTT法检测细胞存活率,丙二醛(MDA)试剂盒检测MDA的含量,XFp细胞能量代谢分析仪检测细胞线粒体呼吸功能及糖酵解功能。[结果]PM_(2.5)各浓度组SH-SY5Y细胞存活率均低于对照组(均P<0.05);80、160、320mg/LPM_(2.5)组细胞内MDA含量高于对照组(均P<0.05)。PM_(2.5)各浓度组细胞基础耗氧率、ATP偶联的有氧呼吸速率低于对照组(P<0.05),分别下降了13.8%、19.7%、25.1%、35.8%、45.2%和17.0%、21.9%、28.3%、39.2%、47.9%;40、80、160、320mg/LPM_(2.5)组细胞有氧呼吸最大值较对照组下降了19.0%、24.2%、28.5%、40.7%(均P<0.05)。80、160、320mg/LPM_(2.5)组细胞糖酵解水平和糖酵解最大值低于对照组(均P<0.05),分别下降了15.9%、22.1%、26.1%和16.5%、19.6%、21.5%。SH-SY5Y细胞基础耗氧率、ATP偶联的有氧呼吸速率、质子漏耗氧速率、有氧呼吸最大值、糖酵解水平及糖酵解最大值与细胞存活率呈正相关(r在0.65~0.86之间,P<0.01),与MDA值呈负相关(r为-0.53^-0.86,P<0.05或P<0.01)。[结论]PM_(2.5)可引起SH-SY5Y细胞的氧化损伤,降低细胞线粒体呼吸功能及糖酵解功能。[Objective] To study the effect of atmospheric fine particulate matters （PM2.5） on the energy metabolism of human neuroblastoma （SH-SY5Y） cells.[Methods] SH-SY5Y cells were exposed to different concentrations of PM2.5 （0, 20, 40, 80, 160, 320 mg/L） for 24 h. Cell survival rate was detected by MTT assay, and malondialdehyde （MDA） content with MDA kit, and mitochondrial respiratory function and glycolytic function with XFp extracellular flux analyzer.[Results] The survival rates of SH-SY5Y cells of the PM2.5 groups were lower than that of the control group （Ps 〈 0.05）. The MDA contents of the 80, 160, and 320 mg/L PM2.5 groups were higher than that of the control group （Ps 〈 0.05）. The PM2.5 groups showed lower basal oxygen consumption rate （OCR） and ATP-linked OCR than the control group （OCR reduced by 13.8%, 19.7%, 25.1%, 35.8%, and 45.2%, respectively; ATP-linked OCR reduced by 17.0%, 21.9%, 28.3%, 39.2%, and 47.9%, respectively） （P 〈 0.05）. The 40, 80, 160, and 320 mg/L PM2.5 groups showed lower maximal respiration level than the control group （reduced by 19.0%, 24.2%, 28.5%, and 40.7%, respectively） （Ps 〈 0.05）. The 80, 160, and 320 mg/L PM2.5 groups displayed lower glycolysis and maximum glycolysis than the control group （glycolysis reduced by 15.9%, 22.1%, and 26.1%, respectively; maximum glycolysis reduced by 16.5%, 19.6%, and 21.5%, respectively） （Ps 〈 0.05）. Basal OCR, ATP-linked OCR, proton leak OCR, maximal respiration capacity, glycolysis level, and maximum glycolysis were positively associated with cell survival rate （r ranged from 0.65 to 0.86, P 〈 0.01）, and negatively associated with MDA （r ranged from -0.53 to -0.86, P 〈 0.05 or P 〈 0.01）.[Conclusion] PM2.5 could cause oxidative damage to SH-SY5Y cells with decreasing the mitochondrial respiratory function and glycolysis function.

关 键 词：PM2.5 SH-SY5Y 能量代谢 线粒体呼吸 糖酵解 

分 类 号：R114[医药卫生—卫生毒理学]