射频热疗对膝骨关节炎实验兔血液中白细胞介素-1β及肿瘤坏死因子-α表达的影响  

The effect of radiofrequency-induced hyperthermia on the expression of IL-1 beta and TNF-alpha in the blood of rabbits with knee osteoarthritis

作　　者：傅照华[1] 赵娇娇[3] 曹青青[3] 孔娇 武永飞 张飞[2] Fu Zhaohua;Zhao Jiaojiao;Cao Qingqing;Kong Jiao;Wu Yongfei;Zhang Fei(Department of Rehabilitation Medicine,Inner Mongolia Baogang Hospital,Baotou 014010,China)

机构地区：[1]内蒙古医科大学第三附属医院(内蒙古包钢医院)康复医学科,包头014010 [2]内蒙古医科大学第三附属医院(内蒙古包钢医院)骨科,包头014010 [3]内蒙古医科大学

出　　处：《中华物理医学与康复杂志》2018年第7期481-485,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：内蒙古自治区自然科学基金项目（2013MS1101）

摘　　要：目的探讨射频热疗对兔膝骨关节炎形成过程中关节软骨组织形态学及血清中自细胞介素-10（IL-1β）、肿瘤坏死因子-α[（TNF—α）的影响。方法选取雄性家兔54只，采用改良Hulth造模法将其右后肢制成实验性膝关节骨关节炎模型。待造模成功后，将上述实验兔随机分为模型组、鹿瓜多肽组及射频热疗组。鹿瓜多肽组于制模后给予鹿瓜多肽肌肉注射，射频热疗组于制模后给予射频热疗干预，模型组制模后未给予特殊处理。于治疗7d、13d及19d时每组分别取6只实验兔处死，取右侧股骨内侧髁软骨，采用改良Mankins标准对其进行形态学评分，同时采用ELISA法检测各组实验兔血清中IL-1β及TNF-α含量。结果经相同时间治疗后，发现模型组、鹿瓜多肽组及射频热疗组右侧股骨内侧髁软骨Mankins评分及血清中IL-1β、TNF-α含量均依次降低，组间差异均具有统计学意义（P〈0．05）。射频热疗组随着治疗时间延长，其右侧股骨内侧髁软骨Mankins评分及血清中IL-1β、TNF—α含量均逐渐降低，各观察时间点间差异均具有统计学意义（P〈0．05）。结论射频热疗治疗兔膝骨关节炎的疗效明显优于鹿瓜多肽，其治疗机制可能与调控血清中IL-1β、TNF—α含量有关。Objective To explore the effect of radiofrequency-induced hyperthermia on the morphology of articular cartilage and any changes in serum-1 interleukin-1 （IL-1β） and tumor necrosis factor-alpha （TNF-α） in the process of knee osteoarthritis in rabbits. Methods Fifty-four male rabbits were selected and knee osteoarthritis was introduced to their right hind limbs using the modified Huhh model. They were then randomly divided into a model group, a eervus and cucumis polypeptide （CCP） group and a radiofrequeney thermotherapy （RT） group, each of 18. The CCP group was injected with deer melon peptide intramuscularly. The RT group was given radiofrequency hyperthermia treatment. The model group was not provided with any special treatment. On the 7th, 13th and 19th day of the treatment, 6 rabbits in each group were sacrificed to resect the right medial femoral condyle cartilage. The morphological characteristics of the cartilage were evaluated using the modified Mankins score, while the content of IL-1β and TNF-α in the serum were detected using enzyme-linked immuno-sorbent assays （ELISAs）. Results At the same time points, the average Mankins score and the average content of IL-1β and TNF-α in the serum of the model group were significantly higher than in the CCP group, with the values in the latter group significantly higher than in the RT group. In the RT group, the average Mankins score, as well as the IL-1 beta and TNF-alpha levels in the serum, decreased significantly with longer treatment. Conclusion Radiofrequeney-indueed hyperthermia is superior to deer melon polypeptide in treating knee osteoarthritis, at least in rabbits. Its therapeutic mechanism may be related to the control of serum IL-1 beta and TNF-alpha levels.

关 键 词：膝骨关节炎 射频热疗 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R684.3[医药卫生—骨科学]