三苯氧胺对乳腺癌细胞生长抑制、凋亡诱导作用及机制研究  

Effect of growth inhibition and apoptosis induction of tamoxifen on the breast cancer cell and its mechanism

作　　者：吴涛[1] 陆明[1] WU Tao,LU Ming(Department of Oncology, Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University, Urumqi 830000, Chin)

机构地区：[1]新疆医科大学附属中医医院肿瘤一科,乌鲁木齐830000

出　　处：《中国临床药理学杂志》2018年第14期1649-1651,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究三苯氧胺对乳腺癌MCF-7细胞生长及凋亡的影响及其作用机制。方法取对数生长期MCF-7细胞,低、中、高剂量实验组分别给予1,2.5,5μg·mL^(-1)三苯氧胺处理,对照组细胞则以等量生理盐水,分别继续培养24,48,72 h。用噻唑蓝(MTT)法及流式细胞术分别检测乳腺癌MCF-7细胞增殖抑制及凋亡情况;用蛋白免疫印迹法检测各组乳腺癌MCF-7细胞存活素(survivin)蛋白表达情况;光学显微镜下观察对照组及低剂量实验组细胞干预72h后细胞形态学变化。结果对照组和低、中、高剂量实验组干预72 h时乳腺癌MCF-7细胞生长抑制率分别为(22.56±2.96)%,(53.45±3.85)%,(54.21±2.89)%,(55.02±3.02)%;乳腺癌MCF-7细胞凋亡率分别为(4.21±1.38)%,(13.55±1.56)%,(13.86±1.57)%,(14.03±1.61)%。与对照组比较,实验组乳腺癌MCF-7细胞生长抑制率及凋亡率均显著升高(P<0.01),但低、中、高剂量实验组间差异无统计学意义(P>0.05);实验组乳腺癌MCF-7细胞生长抑制率及凋亡率随培养时间的延长呈逐渐升高趋势(P<0.01)。干预24 h后,对照组及低剂量实验组乳腺癌MCF-7细胞survivin蛋白表达量分别为1.09±0.03,0.85±0.02;干预48 h分别为1.11±0.03,0.76±0.02;干预72 h分别为1.12±0.02,0.71±0.01,差异均有统计学意义(均P<0.01)。结论三苯氧胺可显著抑制乳腺癌MCF-7细胞生长,并诱导其凋亡,其作用机制可能与下调乳腺癌MCF-7细胞survivin蛋白表达相关。Objective To explore the effect of growth inhibition,apoptosis induction of tamoxifen on the breast cancer MCF-7 cells and its mechanism. Methods The logarithmic phase MCF-7 cell in experimental-L/M/H groups were treated with 1,2. 5,5 μg · mL^-1 tamoxifen,the cells in control group were treated with equivalent normal saline. All groups were treated for 24,48,72 h sequentially. The growth inhibition and apoptosis of breast cancer MCF-7 cell were detected by methylthiazolyldiphenyl-tetrazolium（ MTT） method and flow cytometry;the expression of survivin protein apoptosis of breast cancer MCF-7 cell in each group was detected by Western Blot,the changes of cell morphology in control group and experimental-L group at 72 h after managed were observed under optical microscope. Results The growth inhibition rates of control group and experimental-L/M/H groups were（ 22. 56 ± 2. 96） %,（ 53. 45 ± 3. 85） %,（ 54. 21 ± 2. 89） %,（ 55. 02 ± 3. 02） %; the apoptosis rate were（ 4. 21 ± 1. 38） %,（ 13. 55 ± 1. 56） %,（ 13. 86 ± 1. 57） %,（ 14. 03 ± 1. 61） %. Compared with control group,the growth inhibition rate and apoptosis rate of breast cancer MCF-7 cell in experiment group increased significantly（ P〈0. 01）,while there was no significant difference among experimental-L/M/H groups（ P〉0. 05）; the growth inhibition rate and apoptosis rate of apoptosis rate of breast cancer MCF-7 cell in experiment groups showed a trend of increasing gradually with the lengthen of action time（ P〈0. 01）. The expression of survivin protein in control group and experimental-L group at 24 h after managed were 1. 09 ± 0. 03,0. 85 ± 0. 02; and were 1. 11 ± 0. 03,0. 76 ± 0. 02 at 48 h after managed; and were 1. 12 ± 0. 02,0. 71 ± 0. 01 at 72 h after managed,all with significant difference（ all P〈0. 01）. Conclusion Tamoxifen can inhibit the growth of breast cancer MCF-7 cell,induce its apoptosis,and the action mechanism maybe related to down-regulation of survivin protein of breast canc

关 键 词：乳腺癌 MCF-7细胞 三苯氧胺 SURVIVIN蛋白 

分 类 号：R979.1[医药卫生—药品]