绞股蓝籽油食品安全毒理学评价及抗衰老研究  

Food safety toxicology evaluation and anti-aging analysis of Gynostemma pentaphyllum seed oil

作　　者：杜楠[1,2] 王璐 白鸽[3] 张明星 肖娅萍[1,2] 张坤 王攀[3] 王筱冰[3] 刘全宏[1,3] DU Nan;WANG Lu;BAI Ge;ZHANG Mingxing;XIAO Yaping;ZHANG Kun;WANG Pan;WANG Xiaobing;LIU Quanhong(Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,Xi＇an,Shaanxi 710062,Chinas;National Engineering Laboratory for Developing of Endangered Chinese Crude Drugs in Northwest of China,Xi＇ an,Shaanxi 710062,China;College of Life Science,Shanxi Normal University,Xi＇ an,Shaanxi 710062,China)

机构地区：[1]药用植物资源与天然药物化学教育部重点实验室,陕西西安710062 [2]西北濒危药材资源开发国家工程实验室,陕西西安710062 [3]陕西师范大学生命科学院,陕西西安710062

出　　处：《西北农林科技大学学报（自然科学版）》2018年第5期131-140,148,共11页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十二五"科技支撑计划项目(2011BAI06B05)

摘　　要：【目的】对绞股蓝籽油(GPSO)进行毒理学分析与评价,为绞股蓝籽油的生物学功能研究及其新资源的开发提供科学依据。【方法】通过小鼠急性经口毒性试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验,对GPSO进行食品安全毒理学评估。在小鼠急性经口毒性试验中,于小鼠禁食16h后,各试验组分别经口一次性灌胃0,4.6,10.0及21.5g/kg GPSO,连续观察14d,记录小鼠的饮食、运动、排泄、中毒表现及死亡情况;在小鼠骨髓嗜多染红细胞微核试验中,试验组小鼠以2.5,5.0,10.0g/kg的剂量分别灌胃GPSO,空白对照组以环磷酰胺为阳性药物腹腔注射给药,每天1次,持续5d,处死后取骨髓制作骨髓细胞涂片,镜下观察并计算微核率;在小鼠精子畸形试验中,以性成熟雄性小鼠为试验动物,其中试验组小鼠以2.5,5.0,10.0g/kg的剂量分别灌胃GPSO,空白对照组以环磷酰胺为阳性药物腹腔注射给药,每天1次,持续5d,在最后1次灌胃7,14,21,28和35d后分别处死小鼠,取附睾制作精子涂片,镜检并计算精子畸形率。通过注射D-半乳糖建立衰老模型,同时以绞股蓝籽油作为抗衰老试验药物,按2.0,3.0,4.0mL/kg的剂量进行灌胃给药,60d后检测小鼠体质量、各脏器指数及血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)浓度,以及小鼠肝脏、脑组织中超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,并观察小鼠肝脏组织的病理变化。【结果】绞股蓝籽油对雌雄小鼠急性经口半数致死剂量LD50值大于10.0g/kg,依据急性毒性剂量分级标准属于实际无毒物质;小鼠骨髓嗜多染红细胞微核试验表明,绞股蓝籽油3个剂量组中雌雄小鼠骨髓嗜多染红细胞微核率为2.0‰~3.8‰,与空白对照组相比无显著差异(P>0.05),证明绞股蓝籽油不会对小鼠骨髓嗜多染红细胞微核数造成显著影�【Objective】This study evaluated the toxicology of Gynostemma pentaphyllum seeds oil（GPSO）to provide basis for studying biological function of GPSO and developing new resources.【Method】Mice acute oral toxicity test,mouse bone marrow polychromatic erythrocyte micronucleus test,and mouse sperm deformation test were conducted to evaluate the food safety toxicology.For mice acute oral toxicity test,after fasting for 16 h,each group of mice was orally administered with 0,4.6,10.0 and 21.5 g/kg of GPSO.Then the mice were continuously observed for 14 d,and the diet,exercise,excretion,poisoning performance and death were recorded.For mouse bone marrow polychromatic erythrocyte micronucleus test,the mice in experimental groups were treated with 2.5,5.0 and 10.0 g/kg of GPSO while the blank control group was injected intraperitoneally with cyclophosphamide as a positive drug once a day for 5 days.Bone marrow cells were sacrificed,the incidence of micronuclei was observed and calculated under microscope.For mouse sperm deformation test,male mice were selected and treated with 2.5,5.0 and 10.0 g/kg of GPSO while the blank control group was injected intraperitoneally with cyclophosphamide as a positive drug once per day for 5 days.Mice were sacrificed at the last time after 7,14,21,28 and 35 days,epididymis were taken to produce sperm smears for microscopy observation and calculating sperm deformity rate.The food safety toxicological evaluation of GPSO was carried out by above three tests.D-galactose was injected to establish an aging model while the GPSO was administered as an anti-aging test drug at doses of 2.0,3.0,and4.0 mL/kg.After 60 days,mice organ index and levels of total cholesterol（TC）,triglyceride（TG）,low density lipoprotein（LDL）and high density lipoprotein（HDL）in serum were determined.The activities superoxide dismutase（SOD）,total antioxidant capacity（T-AOC）,catalase（CAT）activity and malondialdehyde（MDA）in liver and brain tissues of mice were observed and the pathologica

关 键 词：绞股蓝籽油 食品安全性评价 毒理学分析 抗衰老作用 D-半乳糖 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.6[轻工技术与工程—食品科学与工程]