团头鲂和草鱼肠道纤维素酶产生菌的筛选和鉴定  

Screening and identification of cellulase-producing strains from intestines of Megalobrama amblycephala and Ctenopharyngodon idellus

作　　者：王魁云 蒋霞云[1] 江小妹 邹曙明[2] WANG Kuiyun;JIANG Xiayun;JIANG Xiaomei;ZOU Shuming(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Aquatic Genetic Resources and Utilization Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海海洋大学农业部种质资源与利用重点开放实验室,上海201306

出　　处：《上海海洋大学学报》2018年第4期624-632,共9页Journal of Shanghai Ocean University

基　　金：国家自然科学基金(31201760;31572220)

摘　　要：采用以羧甲基纤维素(CMC)为唯一碳源的选择性培养基,通过直接筛选和富集分离纯化再筛选的初筛方法,透明圈法和摇瓶发酵的复筛方法,从团头鲂和草鱼肠道中,筛选到2株具有较高纤维素酶活性的纤维素酶产生菌MA35和MC。16S r DNA/ITS分子鉴定和形态学观察分析表明:来源于团头鲂的真菌MA35鉴定为雪白曲霉(Aspergillus niveus),来源于团头鲂的细菌MA1和来源于草鱼的细菌CI10为同一种细菌MC,鉴定为维氏气单胞菌(Aeromonas veronii)。发酵趋势的研究表明:在发酵过程中,与MC相比,MA35具有更高的胞外和胞内纤维素酶活力。本研究不仅从草鱼肠道内筛选到了纤维素酶产生菌,而且从我国特有的水生生物团头鲂肠道内分离到了一株新的纤维素酶产生菌,为草食性鱼的合理性养殖和鱼类饲料的膳食性配比提供了科学的研究基础。Using the selective medium with carboxymethyl cellulose（CMC） as the sole carbon source,through direct screening and screening after enrichment,separation,purification as the preliminary screening methods,and the transparent circle method and shake flask fermentation as the second screening methods,two strains of cellulase-producing microorganisms with higher cellulase activity, MA35 and MC, were screened out from intestines of Megalobrama amblycephala and Ctenopharyngodon idellus. 16 S r DNA/ITS molecular identification and morphological observation showed that： MA35 from Megalobrama amblycephala belonged to Aspergillus niveus,MA1 from Megalobrama amblycephala and CI10 from Ctenopharyngodon idellus were actually identical bacteria,MC,belonged to Aeromonas veronii. Fermentation trend studies indicated that MA35 had a higher extracellular and intracellular cellulase activity compared to MC. This study not only screened out cellulase-producing strains from the intestines of Ctenopharyngodon idellus,but also isolated a new cellulase-producing strain from Chinese unique aquatic organism Megalobrama amblycephala,providing a scientific research basis for the rational aquaculture of herbivorous fish and the dietary ratio of fish feed.

关 键 词：草食性鱼 纤维素酶产生菌 筛选 鉴定 

分 类 号：Q939[生物学—微生物学] S917.1[农业科学—水产科学]