pH值对剩余污泥微氧水解酸化溶出物及微生物群落结构的影响  

Effect of p H Values on Hydrolysis and Acidfication of Waste Activated Sludge and Microbial Community Structures at Microaerobic Conditions

作　　者：曾薇[1,2] 郭京京 纪兆华 赵丹[1] 彭永臻[1] ZENG Wei;GUO Jingjing;JI Zhaohua;ZHAO Dan;PENG Yongzhen(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Beijing University of Technology,Beijing 100124,China;Jiangsu Tianyu Environmental Protection Group Co.,Ltd.,Yangzhou 225200,China)

机构地区：[1]北京工业大学“城镇污水深度处理与资源化利用技术”国家工程实验室,北京100124 [2]江苏天雨环保集团有限公司,江苏扬州225200

出　　处：《应用基础与工程科学学报》2018年第3期471-482,共12页Journal of Basic Science and Engineering

基　　金：国家自然科学基金资助项目(51578016);国家重点研发计划(2016YFC0401103)

摘　　要：将剩余活性污泥消化产物用作污水脱氮除磷的碳源,可以在污泥减量化的同时,解决碳源不足的问题,将无害化处理与资源化利用并举.本研究考察了微氧曝气条件下p H值对污泥水解酸化溶出物及微生物群落结构的影响.研究结果表明,碱性条件下,污泥微氧水解酸化溶出物中VFAs（Volatile fatty acids）、可溶性COD、蛋白质和多糖浓度均高于酸性条件,最佳p H值为10.0,TVFA（Total volatile fatty acid）浓度最高达到4 156.4mg COD/L,乙酸是主要产物,占TVFA的52%,蛋白质浓度是多糖浓度的3~4倍;碱性及微曝气条件下,氨氮的溶出浓度远小于酸性条件.研究结果证实了污泥微氧消化开发内碳源的可行性,原污泥和p H值4.0的酸性条件下,变形菌门（Proteobacteria）是优势菌种,分别占59.9%和38.6%;p H10.0的碱性条件下,厚壁菌门（Firmicutes）转变为为优势菌种,占70.9%,其中隶属于Firmicutes的梭菌纲（Clostridia）所占比例最大,约为63.4%.不同酸碱条件下,微生物的群落结构及优势菌种的改变导致不同的污泥消化性能.Reduction and reclamation of waste activated sludge can be achieved through digested sludge supernatant as a carbon source for biological nutrient removal from wastewater. Under microaerobic conditions,the effect of p H values on hydrolysis and acidfication of sludge and microbial community structures was investigated in this study. Results indicate that at alkalic conditions,the concentrations of volatile fatty acids（ VFAs）,soluble COD,protein and polysaccharide in digestion supernatant are higher than those at acidic conditions. The optimal p H value is 10. 0,The maximal total volatile fatty acid（ TVFA） concentration reaches4 156. 4 mg COD/L,among which acetic acid is the main component,accounting for 52% of TVFA. Protein content is three to four times of polysaccharide. Ammonia concentrations under alkalic and microaerobic conditions are much lower than those at acidic conditions. The outcomes suggest digested sludge supernatant as a carbon source for biological nutrient removal is feasible. Proteobacteria was dominant at raw sludge and pH of 4. 0,with a percentage of59. 9% and 38. 6%,respectively; Whereas Firmicutes become dominant at p H of 10. 0,accounting for 70. 9%. The percentage of Clostridia affiliated with Firmicutes is the highest,up to 63. 4%. At different p H values,changes of microbial community structures and dominant species lead to the difference of sewage sludge microaerobic digestibility.

关 键 词：剩余活性污泥 微氧消化 PH值 水解酸化 微生物群落结构 

分 类 号：X703[环境科学与工程—环境工程]