罂粟药材质量标准研究  

Study on Quality Standard of Papaver Somniferum L.

作　　者：轩辕欢[1] 蔡明江[2] XUAN Yuan-huan;CAI Ming-jiang(Department of Pharmacy,Xinjiang Medical University Affiliated Hospital of Traditional Chinese Medicine,Urumqi 830004,China;Department of Endocrinology,Armed Police Xinjiang General Team Hospital,Urumqi 830004,China)

机构地区：[1]新疆医科大学附属中医医院药学部,新疆乌鲁木齐830004 [2]武警新疆总队医院内分泌科,新疆乌鲁木齐830004

出　　处：《药物生物技术》2018年第3期233-236,共4页Pharmaceutical Biotechnology

摘　　要：针对罂粟药材进行质量标准研究,为罂粟药材规范合理的发展提供理论依据。参照2015年版《中国药典》附录显微鉴别、水分、总灰分、薄层、浸出物(水溶性及醇溶性)等检测方法,含量测定方法采用色谱柱为Agilent TC-C18(4.6 mm×150 mm,5μm);流动相为乙腈-0.5%醋酸铵溶液(含三乙胺0.08%)25∶75;检测波长287 nm下对吗啡进行定量分析,10批次药材水分平均值3.89%、灰分平均值为7.11%、醇不溶性灰分平均0.51%、水和乙醇热浸测定平均值9.30%和5.82%;薄层色谱法(TLC)对罂粟中吗啡成分进行鉴别,TLC斑点清晰,专属性强;HPLC测定了10批药材中的吗啡含量在0.010 8~0.046 9 mg/g之间,平均值为0.035 43 mg/g。试验为药材的质量检测提供了充分的数据,薄层TLC鉴别斑点清晰、重现性好,显微鉴别有明显特征,为该药材质量可控提供鉴定基础。To study the quality standard of papaver,and to provide a theoretical basis for the rational development of opium,the contents of microscopic identification,moisture content,total ash content,content determination（ HPLC）,water soluble and alcohol soluble extract were detected according to Chinese Pharmacopoeia. The result was analyzed by TLC in morphine poppy ingredients and TLC spots were clear and reproducible; an average value of water was 3. 89%,with an average of 7. 11% ash,acid insoluble ash,an average of 0. 51% water and ethanol extraction determination of average values of 9. 30% and 5. 82%. HPLC was used to determine in 10 batches of morphine content in 0. 0108 ~ 0. 046 9 mg/g between the average value of 0. 035 43 mg/g. The quality of medicinal materials provided sufficient data. Thin layer TLC identification spots were clear and reproducible,and microscopic identification had obvious characteristics,providing the identification basis for the quality control of the medicinal materials.

关 键 词：罂粟 质量标准 吗啡 高效液相色谱 薄层色谱 浸出物 

分 类 号：R971.2[医药卫生—药品]