白桦BpTCPs基因家族生物信息学及时空表达分析  

Bioinformatics and expression analysis of BpTCPs in Betula platyphylla Suk.

作　　者：董京祥[1] 任丽 张园 杨洋[1] 黄海娇 李慧玉[1] DONG Jingxiang;REN Li;ZHANG Yuan;YANG Yang;HUANG Haijiao;LI Huiyu(School of Forestry,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《南京林业大学学报（自然科学版）》2018年第4期113-118,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家重点研发计划(2017YFD0600603)

摘　　要：【目的】通过研究白桦TCP转录因子的序列特征及其在不同时期组织中的表达规律,为揭示BpTCPs在白桦生长发育中的作用提供证据。【方法】依据白桦45个转录组测序结果,共获得15条TCPs基因。利用生物信息学方法对TCPs基因进行了全面分析,采用实时荧光定量PCR技术分析基因在不同组织中的表达模式。【结果】生物信息学分析发现,15条BpTCPs均含有1个高度保守的b HLH结构域,系统进化分析发现15条白桦TCP分属于TCP蛋白的两大类;qRT-PCR分析结果显示,自5月到9月的生长阶段中,顶芽中BpTCPs的表达水平呈现不同程度升高的变化趋势;茎中BpTCP3在整个生长季上调表达;BpTCP4、BpTCP10及BpTCP7、BpTCP8分别在越冬后的雌花及雄花中上调表达。【结论】明确了BpTCPs基因的功能及揭示其参与调控植物生长的分子机制奠定基础。【Objective】To analyze the sequence features and gene expression patterns of TCP transcription factors from Betula platyphylla Suk. in different tissues and time points. 【Method】Based on 45 birch transcriptome databases,15 BpTCP genes were identified and the sequence features and gene expression at different time points and in different tissues（ leaves,terminal buds,stems,male inflorescences and female inflorescences） were analyzed.【Result】Bioinformatic analysis showed that all 15 BpTCPs contained a highly conserved b HLH domain. Phylogenetic analysis showed that the 15 birch TCP proteins belonged to two major categories.The qRT-PCR results suggested that the expression levels of the 15 BpTCP genes changed significantly in terminal buds and that most BpTCP genes were up-regulated from May to September. In stems,BpTCP3 exhibited an increasing pattern during the full growth period; BpTCP4,BpTCP10,BpTCP7 and BpTCP8 were up-regulated in male and female inflorescences after winter.【Conclusion】This study may provide information for further research on the functional characteristics of BpTCPs gene and the molecular mechanism（ s）involved in the regulation of plant growth.

关 键 词：白桦 TCP基因家族 生物信息学 基因表达 

分 类 号：S792[农业科学—林木遗传育种] Q781[农业科学—林学]