苗药理气活血滴丸对心肌缺血再灌注大鼠左心室肌细胞损伤的防护作用  

Protective Effect of Liqihuoxue Dripping Pill on Left Ventricular Myocytes Injury in Rats with Myocardial Ischemia and Reperfusion

作　　者：李尧锋[1] 陈向云[1] 彭芳[1] 罗竹 徐剑[1] 陈云志[1] 田紫平 熊仕芳 杨长福[1] 张永萍[1] Li Yaofeng;Chen xiangyun;Peng Fang;Luo Zhu;Xu Jian;Chen Yunzhi;Tian Ziping;Xiong Shifang;Yang Changfu;Zhang Yongping(Guiyang college of Traditional Chinese Medicine,Guiyang 550025;Guizhou Yibai Pharmaceutical Co.,Ltd.,Guiyang 550008)

机构地区：[1]贵阳中医学院,贵阳550025 [2]贵州益佰制药股份有限公司,贵阳550008

出　　处：《中药药理与临床》2018年第3期157-161,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：贵州省科技厅重点项目(黔科合基础[2016]1401);贵州省科技厅合作计划项目(黔科合LH字[2016]7120);贵州省中管局项目(QZYY-2016-015);贵州省教育厅项目(黔教合KY字[2016]186)

摘　　要：目的:研究苗药理气活血滴丸对心肌缺血再灌注损伤(MIRI)大鼠心肌的防护机制。方法:将SD大鼠随机分为假手术组、MIRI模型组、理气活血滴丸(175 mg/kg)组和辛伐他汀(40 mg/kg)组。给药组连续灌胃10天,假手术组和MIRI模型组大鼠灌胃等量的生理盐水。末次给药45 min后采用结扎冠状动脉左前降支方法复制MIRI大鼠模型。复灌后监测左心功能;取左心室利用透射电镜观察心肌细胞超微结构;TUNEL-POD法检测心肌细胞凋亡;荧光素酶法检测心肌组织ATP含量;提取心肌细胞线粒体;硫代巴比妥酸法测定心肌细胞线粒体丙二醛(MDA)含量;WST-8法测定线粒体超氧化物歧化酶(SOD)活性情况。结果:与MIRI模型组比较,理气活血滴丸(175 mg/kg)组大鼠左心功能和左心室肌细胞超微结构明显改善;线粒体损伤减轻;细胞凋亡发生率减少;心肌组织ATP含量升高;心肌细胞线粒体MDA含量降低;SOD活性升高。结论:理气活血滴丸对MIRI模型大鼠的防护机制与其改善左心室肌细胞超微结构、抑制细胞凋亡和减轻线粒体氧化损伤有关。Objective： To study the protective mechanism of Liqihuoxue dripping pill against myocardial ischemia reperfusion injury（MIRI） in rats. Methods： Sprauge-Dawley rats were randomized into four groups,including the sham group,MIRI model group,Liqihuoxue dripping pill（175 mg/kg） group and simvatatin（40 mg/kg） group. Rats in each group were intragastrically given drugs for 10 days,once a day.Rats in the sham group and model group were given the same amount of saline. MIRI rat model was established by ligating the left anterior descending coronary artery 45 minutes after gavage. Left ventricular function was observed after ischemia-reperfusion,and left ventricles were collected. The ultrastructure of the cardial myocytes was observed. Apoptosis of myocardial cells was detected by TUNEL. ATP level of myocardial cells was determined by luciferase method. After the mitochodria dissociated,MDA content in the mitochondria was measured by thiobabituric acid assay,and the activity of superoxide dismutase（SOD） was measured by WST-8. Results： The left ventricular function and left ventricular myocytes ultrastructure were significantly improved in Liqihuoxue dripping pill group compared with those in the model group. Moreover,mitochonodrial injury was obviously ameliorated,the incidence of apoptosis was significantly reduced and the content of ATP in myocardium tissues was obviously high,compared with those in the model group,respectively. The content of MDA in myocardial cells mitochondria was decreased and the activity of SOD was increased. Conclusion： The protective mechanism of Liqihuoxue dripping pill on MIRI model rats is related to improving the ultrastructure of left ventricular myocytes,inhibiting apoptosis and mitigating mitochondrial oxidative damage.

关 键 词：理气活血滴丸 大鼠 心肌缺血再灌注损伤 线粒体 细胞凋亡 

分 类 号：R29[医药卫生—民族医学]