百事乐胶囊对抑郁模型大鼠海马隔颞轴神经再生的影响  

Effects of Baishile Capsule on Nerve Regeneration in Hippocampal Septo-temporal Axis of Depressive Rats

作　　者：孟盼[1,2] 杨琴 杨蕙[3] 朱青[1,2] 杜青[4] 张秀丽 雷昌 王宇红[1,2] Meng Pan;Yang Qin;Yang Hui;Zhu Qing;Zhang Xiuli;Lei Chang;Wang Yuhong(Hunan university of traditional Chinese medicine,Changsha 410208;Training Bases,Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry,Changsha 410208;First Hospital of Hunan University of Chinese Medicine,Changsha 410007)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,长沙410208 [3]湖南中医药大学第一附属医院,长沙410007 [4]湖南省中医药研究院,长沙410006

出　　处：《中药药理与临床》2018年第3期169-172,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金青年项目(81403387);国家科技重大专项课题(2017ZX09309026);中医内科学省部共建教育部重点实验室开放基金(ZYNK201503);湖南省教育厅优秀青年资助项目(17B201)

摘　　要：目的:探讨百事乐胶囊对慢性应激抑郁模型大鼠海马隔颞轴神经再生的影响。方法:将50只雄性SD大鼠随机分为空白对照组、抑郁模型组、氟西汀(5.4 mg/kg)组、百事乐胶囊高、低剂量(2.88、0.72 g/kg)组,每组10只。采用慢性不可预见性温和应激建立抑郁大鼠模型,连续应激21天,每组一半的大鼠在造模前腹腔注射Brd U(75mg/kg),另一半大鼠在处死前2h腹腔注射Brd U(100mg/kg),采用糖水偏好实验测试大鼠的抑郁样行为;采用免疫荧光双染技术观察大鼠海马隔颞轴Brdu、Brdu+Neu N、Brdu+DCX、Brdu+GFAP蛋白表达的变化。结果:与空白组比较,模型组大鼠糖水偏食度显著降低;隔颞轴Brd U标记的新生神经细胞及增殖细胞数目显著降低,Brdu+Neu N、Brdu+DCX、Brdu+GFAP的细胞数目显著降低;在给予百事乐胶囊高剂量(2.88 g/kg)干预后,模型大鼠的糖水偏食度显著增加;隔颞轴Brd U标记的新生神经细胞及增殖细胞数目显著升高;Brdu+Neu N、Brdu+DCX、Brdu+GFAP的细胞数目显著升高。结论:百事乐胶囊(2.88 g/kg)能够增加海马隔颞轴新生细胞和增殖细胞的数目,并能显著促进海马隔颞轴神经再生。Objective： To investigate the effect of Baishile capsule on nerve regeneration in hippocampal septo-temporal axis of depressive rats.Methods： 50 SD rats were randomly divided into the control group,depression model group,fluoxetine（5. 4 mg/kg） group and high,medium and low doses of Baishile capsule（2. 88、0. 72 g/kg）. The depression model was established by chronic unpredictable mild stress for 21 days. Half of rats in each group were intraperitoneally injected with Brd U（75 mg/kg） before molding,and the other half were intraperitoneally injected 2 h before the sacrifice. Depressive behaviors of rats were observed by sugar water preference test,and expressions of Brdu,Brdu ＋ Neu N,Brdu ＋ DCX,Brdu ＋ GFAP in hippocampi of rats were detected by immunofluorescence double staining technique. Results：Compared with the control group,the consumption of sugar water in the model group was significantly decreased（P〈0. 01）,the number of cells in Brdu,Brdu ＋ Neu N,Brdu ＋ DCX and Brdu ＋ GFAP significantly decreased（P〈0. 01）. After the intervention of Baishile capsule（2. 88 g/kg）,the consumption of sugar water in model rats significantly increased（P〈0. 05）,the number of new neurons and proliferating cells labeled with Brd U in septo-temporal axis were significantly increased（P〈0. 05）,the number of Brdu ＋ Neu N,Brdu ＋ DCX,Brdu ＋GFAP cells significantly increased（P〈0. 01）. Conclusion： Baishile capsule（2. 88 g/kg） can increase the number of new cells and proliferating cell in hippocampal septo-temporal axis,and significantly promote nerve regeneration of septo-temporal axis in the hippocampus.

关 键 词：百事乐胶囊 抑郁症 隔颞轴 海马 溴脱氧尿苷(BrdU) 

分 类 号：R285.5[医药卫生—中药学]