舒洛地特对脂多糖作用下人腹膜间皮细胞炎症及纤维化的影响  

Effect of sulodexide on lipopolysaccharide induced human peritoneal mesothelial cells inflammation and fibrosis

作　　者：边爱淑 胡晔[1] 杨丽娜[1] 樊怡[1] 马健飞[1] Bian Aishu;Hu Ye;Yang Lina(Department of Nephrology,the First Affiliated Hospital of China Medical University,Shenyang 110001,China;Department of Nephrology,242 Hosiptal of Shenyang City,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院肾内科,辽宁沈阳110001 [2]沈阳二四二医院肾内科,辽宁沈阳110001

出　　处：《国际泌尿系统杂志》2018年第4期624-627,共4页International Journal of Urology and Nephrology

基　　金：国家自然科学基金（81370865）

摘　　要：目的 研究舒洛地特（sulodexide,SLX）对脂多糖（lipopolysaccharide,LPS）作用下人腹膜间皮细胞（human peritoneal mesothelial cells,HPMCs）糖萼组成成分（HA、DCN）和炎症及纤维化因子（IL-6、TGF-β1）表达的影响.方法 将HPMCs随机分为以下各组：对照组;LPS组①不同浓度组：1 ～ 100 μg/mL,LPS作用48 h;②不同时间组：LPS 10 μg/mL作用0～72 h;LPS＋ SLX组：LPS 10μg/mL＋ SLX（50～ 1200 μg/mL）作用48 h.应用ELISA法检测各组上清液中HA、DCN、IL-6及TGF-β1的蛋白表达情况.结果 LPS能刺激HPMCs HA、DCN、IL-6及TGF-β1表达增多,差异具有统计学意义（P＜0.05）.与LPS组相比,SLX作用后能明显上调HA和DCN的表达,明显下调IL-6及TGF-β1的表达.结论 LPS刺激可以上调HPMCs的糖萼表达,并显著上调炎症及纤维化的因子表达;而补充SLX具有修复受损糖萼的作用,并抑制炎症和纤维化,从而保护腹膜功能.Objective To explore the expression of glycocalyx （HA and DCN）,inflammatory and fibrosis factors（IL-6 and TBF-β1） on human peritoneal mesothelial cells（HPMCs） stimulated by lipopolysaccharide.Methods HPMCs were treated on the following conditions：control group and LPS group.①LPS stimulated HPMCs for different concentrations：1-100 μg/mL.②10 μg/mL LPS stimulated HPMCs for different times：0-72 h.③HPMCs were treated with 10 μg/mL LPS and SLX （50-1200 μg/mL） for 48 h.Results Compared to control group,LPS up-regulated the protein of HA,DCN,IL-6 and TGF-β1 with significant difference.Compared to LPS group,treatment of SLX further increased the protein of HA and DCN,down-regulated the expression of IL-6 and TGF-β1.Conclusions LPS up-regulates the protein of glycocalyx,and treatment of SLX repairs glycocalyx through adding GAGs.LPS up-regulates the protein level of inflammatory and fibrosis factors,however,SLX can protect the peritoneal function by inhibiting peritoneal inflammation and fibrosis.

关 键 词：腹膜透析 炎症 纤维化 腹膜 脂多糖类 

分 类 号：R692.5[医药卫生—泌尿科学]