大头典竹扦插过程中营养物质和氧化酶活性变化研究  

Change of Nutrient Content and Oxidase Activities during Cutting Propagation of Dendrocalamopsis beecheyana

作　　者：凡莉莉 薛磊 赖金莉 荣俊冬[1] 魏建文 苏小青[1] 郑郁善[1] FAN Li-li;XUE Lei;LAI Jin-li;RONG Jun-dong;WEI Jian-wen;SU Xiao-qing;ZHENG Yu-shan(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;College of Landscape,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Fujian Chishan State-owned Forest Farm,Dongshan County,Zhangzhou 363000,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学园林学院,福建福州3500023 [3]福建省东山赤山国有防护林林场,福建漳州363000

出　　处：《竹子学报》2018年第1期54-59,共6页Journal of Bamboo Research

基　　金：福建省区域发展项目(2015N3015);福建省农业科技重大专项(2013NZ0001)

摘　　要：以2年生大头典竹(Dendrocalamopsis beecheyana)的粗壮主侧枝为实验材料,采用600 mg·L^(-1)的ABT1溶液预先处理5 min,以m(黄泥土)∶m(泥炭土)=1∶1混合为基质,进行扦插,研究不同扦插时期大头典竹生根特性和扦插生根后可溶性糖、可溶性蛋白和相关抗氧化酶活性变化。结果表明:600 mg·L^(-1)的ABT1处理对扦插生根具有重要影响,处理后的插条生根率最高可达69.84%。大头典竹侧枝扦插生根属于潜伏不定根原基生根型,具体过程分为根的诱导时期(0~14 d)、表达时期(14~21 d)和伸长时期(21~35 d)3个阶段。扦插后,可溶性糖质量分数呈现先降低后上升再下降的变化趋势,可溶性蛋白质量分数则呈现相反变化趋势。在扦插生根过程中氧化酶活性呈规律性变化,过氧化物酶(POD)、吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)活性在不定根诱导时期呈上升趋势;在不定根表达形成期,POD、PPO活性下降,IAAO活性急剧上升,SOD活性保持稳定;在不定根的伸长时期,IAAO和PPO活性下降,POD活性先上升后下降,SOD活性仍维持较高水平。To establish a commercial feasible cutting propagation system, the branches of Dendrocalamopsis beecheyana were used as experimental materials, the cuttings were pretreated with 600 mg · L-1 ABT1 for 5 min, then planted in the mixed substrate of yellow soil and peat （1：1 ）. The rooting characteristics were observed and the contents of soluble sugar, soluble protein and oxidase activities in the cuttings were measured after rooting. The results showed that ABT1 with 600 mg · L-1 had significant effects on rooting of cuttings, and the rooting rate of cuttings reached 69.84%. They rooted from the latent adventitious root primordium, and the rooting process can be divided into induction period （0 -14 d）, expression period （14 -21 d） and elongation period （21 - 35 d）. After plantation of the cuttings, the content of soluble sugar decreased first, then increased, and decreased at last. The content of soluble protein showedthe opposite trend. The activity of peroxidase （POD）, indoleacetic oxidase （IAAO）, Polyphenol oxidase （PPO） and superoxide dismutase （SOD） increased at the root induction stage. During the root formation stage, the activity of POD and PPO decreased, the activity of IAAO increased sharply, and the activities of SOD remained stable. In the root elongation phase, the activities of IAAO and PPO decreased, the activity of POD increased first and then decreased, and the activity of SOD remained high.

关 键 词：大头典竹 扦插生根 营养物质 抗氧化酶 

分 类 号：S795[农业科学—林木遗传育种]