桦树茸对免疫功能低下小鼠免疫调节作用研究  

Immunoregulatory effects of Traditional Chinese medicine huashurong on immunosuppression mice

作　　者：李然 赵伟伟 刘孟安[1] 王斌胜[1] LI Ran ,ZHAO Weiwei ,LIU Meng＇an,WANG Binsheng(College of Integration of Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, P. R. China)

机构地区：[1]滨州医学院中西医结合学院,烟台264003

出　　处：《滨州医学院学报》2018年第4期244-246,250,共4页Journal of Binzhou Medical University

摘　　要：目的研究药用真菌桦树茸对免疫功能低下模型小鼠免疫功能的调节作用。方法 SPF小鼠60只,随机分为6组,每组10只,正常对照(等容生理氯化钠溶液)组、模型(等容生理氯化钠溶液)组、左旋咪唑(40 mg·kg^(-1)·d^(-1))组与桦树茸提取液高、中、低剂量(6 800、3 400、1 700 mg·kg^(-1)·d^(-1))组,灌胃给药,每天1次,连续10 d。腹腔注射环磷酰胺40 mg·kg^(-1)·d^(-1),每天1次,连续3 d,以复制小鼠免疫功能低下模型。细胞计数仪读取白细胞数;酶联免疫吸附(ELISA)法检测血清免疫球蛋白(IgG)水平;称定小鼠胸腺、脾脏与体质量以计算免疫脏器指数。结果与空白组比较,模型组白细胞数减少,血清IgG含量减少,胸腺指数、脾脏指数降低;差异均有统计学意义(P<0.01)。与模型组比较,桦树茸提取物高、中、低剂量组胸腺指数差异有统计学意义(P<0.05),与模型组比较,血清IgG含量增加,白细胞数增加,差异均无统计学意义。结论桦树茸提取物对环磷酰胺所致小鼠免疫功能低下细胞免疫作用具有一定的调节改善作用,对体液免疫以及提升小鼠的白细胞数及IgG含量的作用似有一定的提高作用,但无统计学意义,这可为我们进一步研究桦树茸奠定一定的基础。Objective To investigate the immune regulatory function of tradictional Chinese medicine huashurong on immunosuppression mice induced by cyclophosphamide （ Cy ）. Methods Sixty SPF mice were randomly divided into 6 groups ： blank control groups, levamisole groups （40mg/kg）, model control groups, and high, medium and low-dose huashurong （HSR） groups. Huashurong groups were administrated by gavage at doses of 6 800,3 400,1 700 mg·kg^-1·d^-1to mice for 10 consecutive days, respectively, hnmunosuppressed murine models were established in SPF mice induced by Cy 40 mg/kg body weight once a day for 3 days by intraperitoheal injection on the day in dosing. Cell count meter was used to read white blood cell count. IgG serum levels were detected by ELISA. The mice body weight and spleen weight and thymus weight were analyzed to calculate the organ indexes. Results Compared with the blank group, the model group showed a significant decrease in white blood cell count, IgG serum levels, thymus index and spleen index. Compared with the model group, the HSR groups increased the number of white ceils and thymus index levels. Conclusion HSR extractive has certain effect on the immune regulatory function of immunosuppression mice induced by Cy. HSR extractive could improve the number of white ceils and IgG levels, and has effect on humoral immunity, but the effect was not obvious.

关 键 词：桦村茸提取物 免疫功能低下 细胞免疫 

分 类 号：R285[医药卫生—中药学]