蓝猪耳花粉管在雌蕊生长途径中钙的分布特征  

Distribution of calcium in the stigma,style and pollen tubes of Torenia fournieri during pollen germination and tube elongation

作　　者：陈素红[1,2] 邹璞[2] 廖景平[2] CHEN Su-hong;ZOU Pu;LIAO Jlng-ping(Guangdong Food and Drug Vocational College,Guangzhou 510520;South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

机构地区：[1]广东食品药品职业学院,广州510520 [2]中国科学院华南植物园,广州510650

出　　处：《生物学杂志》2018年第4期39-43,共5页Journal of Biology

基　　金：广东省自然科学基金(2015A030313865)

摘　　要：采用焦锑酸钾沉淀法研究蓝猪耳(Torenia fournieri)花粉管在雌蕊生长途径中钙的分布特征。蓝猪耳花粉从萌发至花粉管生长进入子房过程中,花粉管壁上的钙沉淀颗粒保持较多;管内的钙沉淀颗粒先增多后减少,花粉管进入子房时管内几乎没有钙沉淀颗粒;管外相邻花柱组织的钙沉淀颗粒先增多再减少又增多,直至花粉管进入子房时子房室内有大量的钙沉淀颗粒。蓝猪耳体内生长的花粉管钙也呈梯度分布,且随花粉管不断生长钙梯度分布更加明显。蓝猪耳的花柱未授粉时已出现明显的钙梯度分布特征,并且授粉后花粉管生长到达的部位均有较为丰富的钙。以上结果显示:花粉萌发时,花粉中有足够的钙满足花粉萌发;花粉管生长过程中,钙不断从花柱组织细胞中内流进入花粉管,满足花粉管的生长;花粉管进入子房室,管内无需太多的钙,而管外高钙环境有助于花粉管进入珠孔;蓝猪耳花柱的钙梯度分布对其中生长的花粉管具有引导作用。Potassium antimonite was used to locate loosely bound calcium in the stigma, style and pollen tubes of Torennia fournieri. During pollen germination and tube elongation, the pollen tube wall is densely labeled with calcium precipitates. Calcium precipitates accumulate in the pollen tube, increase in stylar tissue at first and decrease later. When pollen tubes enter into the ovary, there are numerous calcium granules in ovary locule and less in the pollen tube. Calcium gradient exists in in vivo pollen tube of Torenia fournieri and becomes evident along with growth of pollen tube. There is evident calcium gradient in the style of Torennia fournieri before pollination and abundant calcium in the area where pollen tube arrived. It is concluded that there are enough calcium in pollen of Torenia fournieri to supply pollen germination, calcium flows into pollen tube from stylar tissue continuously in order to supply the growth of pollen tube, a higher calcium environment is helpful to pollen tube into the micropyle when pollen tubes grow in ovary locule, the gradient distribution of calcium in the style tissues play a guiding role to the growth of pollen tubes.

关 键 词：蓝猪耳 钙 花粉管 柱头 花柱 

分 类 号：Q945[生物学—植物学]