玉米低磷胁迫诱导型启动子P_(1502)-ZmPHR1在玉米中的瞬时表达  

Transient Expression of Strong Inducible Promoter P_(1502)-ZmPHR1 under Phosphrous Stress Condition in Maize

作　　者：杨瑞娟[1,2] 卢甜 闫蕾 李锐[1] 康晨[1] 常丽芳 张丛卓 白建荣[1] YANG Ruijuan;LU Tian;YAN Lei;LI Rui;KANG Chen;CHANG Lifang;ZHANG Congzhuo;BAI Jianrong(Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;College of Bioengineering,ShanxiUniversity,Taiyuan 030006,China;College of Life Science,Northwest A＆ F University,Yangling 712100,China)

机构地区：[1]山西省农业科学院作物科学研究所,山西太原030031 [2]山西大学生物工程学院,山西太原030006 [3]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《山西农业科学》2018年第8期1231-1234,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省农业科学院农业科技创新项目(ZDSYS1507)

摘　　要：用农杆菌介导法将玉米低磷胁迫诱导型启动子P_(1502)-ZmPHR1与GUS报告基因的融合基因分别转入玉米478未成熟幼胚以及萌动的成熟胚中,通过检测GUS基因的瞬时表达情况来分析该启动子在玉米中的表达活性。结果表明,P_(1502)-ZmPHR1在玉米中低磷条件下被诱导表达,具有启动活性。The fusion gene of strong inducible promoter under phosphrous stress P（1502）-ZmPHR1 in maize and GUS reporter gene was transferred into maize inbreed line 478 immature embryos and mature embryos by Agrobacterium tumefaciens mediated method.Then the transient expression of GUS gene was detected to analyze the expression activity of the promoter in maize. The results showed that P（1502）-ZmPHR1 could be induced and expressed under phosphrous stress conditions in maize.

关 键 词：玉米 P1502-ZmPHR1启动子 瞬时表达 

分 类 号：S512.104[农业科学—作物学]