母树年龄、生长调节剂、容器与基质对崖柏嫩枝扦插的影响  

Effects of the Mother Tree Age,Growth Regulator,Containers and Substrates on Softwood Cutting Propagation of Thuja sutchuenensis

作　　者：秦爱丽[1] 简尊吉 马凡强[1] 郭泉水[1] 郑祥坤 Qin Aili;Jian Zunji;Ma Fanqiang;Guo Quanshui;Zheng Xiangkun(Key Laboratory of Forest Ecology and Environment of State Forestry Administration Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry Beijing 100091;Beijing Bayi School Affiliated Yuquan Middle School Beijing 100091)

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林生态环境重点实验室,北京100091 [2]北京市八一学校附属玉泉中学,北京100091

出　　处：《林业科学》2018年第7期40-50,共11页Scientia Silvae Sinicae

基　　金：国家重点研发计划项目(2016YFC0503103);国家自然科学基金项目(31400474);中央级公益性科研院所基本科研业务费专项资金项目(CAFRIFEEP201401);国家林业局野生动物保护专项(2018)

摘　　要：【目的】研究崖柏母树年龄、生长调节剂、扦插容器、扦插基质等对插穗生根率、不定根数量、根长及根系干质量的影响,探讨影响嫩枝扦插的内在因子和外在环境条件,为崖柏嫩枝扦插繁殖及引种驯化提供参考。【方法】按照正交表L9(34)进行正交试验设计。崖柏嫩枝扦插后70天,调查不同处理及水平下的插穗生根率;扦插后260天,增加对不定根数量、根长及根系干质量的调查,并对其进行统计分析。【结果】母树年龄对插穗生根率和根系发育影响较大。母树年龄越小,插穗的生根率越高。一级不定根数量、一级不定根最长根长和根系干质量的变化与生根率随母树年龄变化的规律基本一致;生长调节剂(IBA)对插穗生根率与根系发育也有较大影响。插后70天的插穗生根率及插后260天的一级不定根数量、一级不定根最长根长和根系干质量均是以IBA(2 000 mg·L-1)速蘸2 min处理的最优;扦插容器类型对插穗生根率的影响不显著,但对一级不定根最长根长影响极显著。优劣次序为:黑色软塑料营养杯>无纺布育苗袋>白色硬塑料营养杯;基质类型对插穗生根率的影响随着扦插后时间的延长逐渐增大,对扦插后70天的插穗生根率影响不显著,对扦插后260天的插穗生根率影响极显著。用草炭土、珍珠岩、蛭石配制的混合基质优于纯草炭土;不同基质对一级不定根最长根长和根系干质量影响显著。【结论】崖柏嫩枝插穗生根率及生根后根系各种指标,不仅受到母树年龄、生长调节剂、扦插容器、扦插基质等因子的单独影响,而且还受各因子的综合作用。随着生根发育阶段的变化,对插穗生根率和根系发育起重要作用的因子也在不断发生变化。对崖柏嫩枝插穗生根率的最佳处理组合为:从3年生母树上采集插穗、用GGR6速蘸处理、无纺布育苗袋、草炭土:珍珠岩:蛭石(1∶2∶1)。对根系�【Objective】A orthogonal experiment on softwood propagation of Thuja sutchuenensis. was carried out to study the effects of different mother tree age,growth regulator,containers and substrates on the rooting rate and the root development indicators such as the number of adventitious roots,length of longest primary adventitious root,root dry mass. The internal and external environmental factors affecting softwood cutting propagation were investigated to provide theoretical and practical basis for the softwood propagation,introduction and domestication of T. sutchuenensis. 【Method】An orthogonal experiment design of four factors and three levels（L9（34）） was applied to study the softwood cutting propagation of T. sutchuenensis in the greenhouse of Chinese Academy of Forestry,Beijing. The rooting rate of cuttings with different treatments was investigated in 70 and 260 days after cutting. The number of adventitious roots,length oflongest primary adventitious root and root dry mass per cutting were measured in 260 days after cutting. The statistical analyses of these indicators were conducted. 【Result】Results indicated that： the age of mother tree had a great impact on the rooting rate and the root development of cuttings. The younger the mother tree,the higher the rooting rate of cuttings.Also,the younger the mother tree,the better the root development. The growth regulator（IBA） had a great impact on the rooting rate and the root development of cuttings. The rooting rate after cutting 70 days and the number of adventitious roots,length of longest primary adventitious root and root dry mass in 260 days after cutting were optimized by IBA（2 000 mg·L-1） dipping in 2 minutes. The containers had little influence on the rooting rate,but it had a significant effect on the length of the longest adventitious root. The order of the merits of various containers was： black soft plastic nutrient cup non-woven fabric nursery bag white hard plastic nutrition cup. The effect of substrates on rooting r

关 键 词：崖柏 嫩枝扦插 母树年龄 生长调节剂 扦插容器 基质 生根率 根系发育 

分 类 号：S723.132.1[农业科学—林木遗传育种]