运输应激牛不同组织HSPs mRNA和蛋白表达研究  

Study on the HSPs mRNA and protein expression of different tissues in transport stressed beef cattle

作　　者：刘延鑫[1,2] 王义翠 孙宇[2] 李业亮[1] 翟磊[2] 郝万清[1] 高腾云[2] LIU Yanxin;WANG Yicui;SUN Yu;LI Yeliang;ZHAI Lei;HAO Wanqing;GAO Tengyun(Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;College of Animal Husbandry and Veterinary Medicine Engineering,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南中医药大学,郑州450008 [2]河南农业大学牧医工程学院,郑州450002

出　　处：《黑龙江畜牧兽医》2018年第13期75-78,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：现代农业产业技术体系建设专项(CARS-37);中国博士后面上基金项目(2012M521396)

摘　　要：为了研究运输应激对牛不同组织热休克蛋白HSPs表达量的影响,试验采用实时荧光定量PCR和Western-blot技术,检测短途公路运输3 h后牛外周血淋巴细胞、心脏、肝脏和背最长肌中HSP27、HSP70和HSP90的表达情况。结果表明：3 h短途运输后,与对照组相比,运输应激组HSPs在不同组织中的表达量显著增加（P〈0.05）。说明HSPs表达量可以作为牛运输应激性状的候选分子指标。The aim of the present study was to demonstrate the expression of heat shock proteins（ HSPs） in different tissues of the beef cattle.The expression of HSP27,HSP70 and HSP90 in peripheral blood lymphocytes,heart,liver and longissimus muscle were detected by real-time fluorescence quantitative PCR and Western-blot. The results showed that compared with the control group,the expression of HSPs in different tissues in the transport stress group was significantly increased in different degrees（ P〈0. 05）. The results suggested that the expression of HSPs could be used as a candidate molecular marker for transport stress traits in cattle.

关 键 词：肉牛 运输应激 热休克蛋白 实时荧光定量PCR 蛋白质印迹 

分 类 号：S813.3[农业科学—畜牧学] S823.2[农业科学—畜牧兽医]