厚朴及其提取物体外抗流感病毒H1N1的初步研究  

Research of Mangnolia Officinalis and its Extractive Against Influenza Virus H1N1 in Vitro

作　　者：周艳萌[1] 李丽 胡芳媛 胡铭 邓文博 ZHOU Yan-meng;LI Li;HU Fang-yuan;HU Ming;DENG Wen-bo(Laboratory of Microbiology and Immunology,Zunyi Medical University,Zunyi.563000,China;Grade 2012 of the Pharmacy,the Medicine ＆ Technology School of Zunyi Medical University,Zunyi 563099,China;Grade 2014 of the Clinical Institu- te,Zunyi Medical University,Zunyi.563000,China)

机构地区：[1]遵义医学院医学微生物与免疫学实验室,遵义563000 [2]遵义医学院医学与科技学院药学系,遵义563000 [3]遵义医学院临床医学系,遵义563000

出　　处：《海峡药学》2018年第7期15-18,共4页Strait Pharmaceutical Journal

摘　　要：目的观察厚朴及其提取物体外抗流感病毒H1N1的作用。方法经鸡胚扩增流感病毒H1N1,并测定其感染MDCK细胞的TCID50;采用MTT法检测药物对细胞的毒性及三种给药方式下药物对病毒的抑制作用,并进一步通过流式细胞仪初步探讨药物抗病毒机制。结果H1N1的TCID50为1.3×10^(-9)·L^(-1);厚朴酚、厚朴水浸液及厚朴水煎液的最大无毒浓度分别为1.2μg·mL^(-1)、10.8mg·mL^(-1)及2400μg·mL^(-1);三种给药方式厚朴水煎液对病毒均无作用,厚朴酚有一定的治疗效果,厚朴水浸液有预防和治疗效果;流式细胞仪结果说明厚朴酚和厚朴水浸液的治疗作用可能是通过调整细胞周期、提高细胞增殖指数而实现的。结论对流感病毒H1N1而言,厚朴酚和厚朴水浸液对其有一定的治疗效果,厚朴水浸液对其有一定的预防作用,而厚朴水煎液对其没有作用。OBJECTIVE To explore the inhibitory effects of Mangnolia Officinalis and its extractive against in- fluenza virus H1 N1. METHODS Propagation of virus in chick embryo and the value of TCID50 were calculated;The MTT test assay was used to evaluate the toxicity and the inhibitory effect of drugs against H1N1 ,and through Flow Cytometry to discuss the antiviral mechanism of Mangnolia Officinalis and its extractive. RESULTS The TCIDso of HI N1 virus to MDCK cells was 1.3 x 10-9 . L-1. The TC0 of Magnolol, Mangnolia Officinalis immersion and decoc- tion in MDCK cells were 1.2μg·mL^（-1）、10.8mg·mL-1and2400μg·mL-1. Three kinds of way of magnolia offici- nalis decoction on virus had no effect. Magnolol had a certain therapeutic effect. Mangnolia Officinalis immersion had prevention and therapeutic effect. Flow cytometry results demonstrated the therapeutic effect of Magnolol and Mangno- lia Officinalis immersion may be improved through the adjustment of the cell cycle and cell proliferation in- dex. CONCLUSION For H1N1 influenza virus, Magnolol and Mangnolia Officinalis immersion had certain thera- peutic effect, Mangnolia Officinalis immersion had certain preventive effect, and magnolia officinalis decoction has no effect.

关 键 词：厚朴 甲型流感病毒 体外 四甲基偶氮唑盐比色法(MTT) 流式细胞仪 

分 类 号：R965[医药卫生—药理学]