A preliminary study on the establishment of an animal model of conjunctivochalasis  

作　　者：Jing-Yun Gan Qing-Song Li Huan-Ming Zhou Wei Zhang Ling-Zhi Lian Zhang Yu Zhen-Yong Zhang 

机构地区：[1]Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine [2]Department of Pathology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine [3]Department of Electron Microscopy, Shanghai Medical College, Fudan University

出　　处：《International Journal of Ophthalmology(English edition)》2018年第6期899-904,共6页国际眼科杂志（英文版）

基　　金：Supported by the Key Medical Discipline Project of Shanghai Municipal Health Bureau-Ophthalmology(No.ZK2015A20);the Health System Independent Innovation Science Foundation of Shanghai Putuo District(No.2015PTKW001);Plateau Science,Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine

摘　　要：AIM： To explore a feasible method on the establishment of an animal model of conjunctivochalasis （CCH）. METHODS： Twelve clean-grade New Zealand white rabbits were divided into four groups （n=3/group）： the control group （one received no interventions, and the others underwent subconjunctival injection of sterile water）, the matrix metalloproteinases （MMPs） group （administered subconjunctival injection of MMP-3）, the aging group （administered subcutaneous injection of D-galactose）, the tumor necrosis factor-α （TNF-α） solution group （administered eye drops of TNF-α）. Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy （TEM） were performed after 12wk. RESULTS： Among all groups, the MMPs group had the following changes： the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers （Trichrome staining） and the broken elastic fibers （Aldehyde-fuchsin staining）; the focal necrosis of fibroblasts （TEM）. CONCLUSION： Administration of MMPs may be a feasible method for the establishment of an animal model of CCH.AIM： To explore a feasible method on the establishment of an animal model of conjunctivochalasis （CCH）. METHODS： Twelve clean-grade New Zealand white rabbits were divided into four groups （n=3/group）： the control group （one received no interventions, and the others underwent subconjunctival injection of sterile water）, the matrix metalloproteinases （MMPs） group （administered subconjunctival injection of MMP-3）, the aging group （administered subcutaneous injection of D-galactose）, the tumor necrosis factor-α （TNF-α） solution group （administered eye drops of TNF-α）. Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy （TEM） were performed after 12wk. RESULTS： Among all groups, the MMPs group had the following changes： the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers （Trichrome staining） and the broken elastic fibers （Aldehyde-fuchsin staining）; the focal necrosis of fibroblasts （TEM）. CONCLUSION： Administration of MMPs may be a feasible method for the establishment of an animal model of CCH.

关 键 词：animal model CONJUNCTIVOCHALASIS EXTRACELLULARMATRIX inflammatory factors matrix metalloproteinase 

分 类 号：R77[医药卫生—眼科]