“森淼”文冠果新品系组织培养技术  

Tissue Culture of Xanthoceras sorbifolia “Senmiao”

作　　者：王娟[1] 王薇 WANG Juan;WAMG Wei(International Forestry Cooperation Program Administration Center,Forestry Department of Shaanxi,Xi＇an,Shaanxi 710082;Shaanxi Forestry Education and training Station,Xi＇an,Shaanxi 710082)

机构地区：[1]陕西省林业国际合作项目管理中心 [2]陕西省林业教育培训站,西安710082

出　　处：《陕西林业科技》2018年第3期7-10,14,共5页Shaanxi Forest Science and Technology

摘　　要：以文冠果新品系"森淼"休眠枝水培嫩枝为外植体,研究了消毒时间对启动培养、植物生长调节剂对增殖培养与生根培养的影响,以建立一种启动培养污染率低、增殖系数与生根率相对较高的"森淼"新品系组织培养体系。结果表明:以休眠枝水培嫩枝为外植体可从源头控制启动培养的污染率,0.1%升汞消毒8min可将污染率控制在10%以内;在MS附加1.0mg·L^(-1)6-BA以及0.5mg·L^(-1) IBA的培养基中外植体腋芽萌发快,萌发率高;MS附加2.0mg·L^(-1) 6-BA以及0.5mg·L^(-1) IBA的培养基适合"森淼"品系无菌芽增殖,平均每个外植体增殖芽数为3.7;1/2MS附加0.5mg·L^(-1) IBA的培养基适合"森淼"品系无菌芽生根,生根率可达91.4%,NAA较IBA的生根效果差。Effects of sterilization time on preliminary culture and plant growth regulators on enrichment and rooting culture were studied using fresh branches from dormant branches of Xanthoceras sorbifolia ＂Senmiao＂,a new variety,as explants.The tissue culture system with low contamination rate in preliminary culture stage and high proliferation and rooting rate was established.The result showed that fresh branches from dormant branches of Xanthoceras sorbifolia as explants can control contamination effectively at their source.Contamination rate can be controlled within 10%through 8 minutes of disinfection with 0.1% HgCl2.Axillary buds can germinate rapidly on the MS medium with1.0 mg·L-1 6-BA and 0.5 mg/L IBA.MS medium with 2.0 mg/L 6-BA and 0.5 mg·L-1 IBA is suitable for enrichment culture of germ-free shoots and average shoot number from each explant is3.7 on this medium.1/2 MS medium with 0.5 mg/LIBA is suitable for rooting culture of germ-free shoots.The rooting rate is up to 91.4% on this medium.Rooting effect of IBA precede that of NAA.The results will promote rapid propagation and molecular breeding of Xanthoceras sorbifolia ＂Senmiao＂,a oilseed variety of trees.

关 键 词：文冠果 休眠枝 水培 组织培养 

分 类 号：S723.132.6[农业科学—林木遗传育种]