Bax和Bcl-2在增生性瘢痕中的表达特征及其意义  

Characteristics of Bax and Bcl-2 expression in hypertrophic scars and its biological significance

作　　者：陈伟[1] 付小兵[1] 孙同柱[1] 孙晓庆[1] 赵志力[1] 盛志勇[1] 

机构地区：[1]解放军第304医院创伤外科研究室,北京100037

出　　处：《创伤外科杂志》2002年第5期276-278,共3页Journal of Traumatic Surgery

基　　金：国家重大基础研究规划资助项目(G1999054204);国家杰出青年科学基金(39525024);国家自然科学基金(30170996)

摘　　要：目的　观察Bax和Bcl 2蛋白在增生性瘢痕和正常皮肤组织内的表达特征及其对增生性瘢痕形成的影响。方法　用免疫组化方法和常规病理技术确定这两种蛋白在 8例增生性瘢痕和 8例正常皮肤中的表达变化规律。结果　在正常皮肤内 ,Bax主要存在于表皮基底层细胞和部分成纤维细胞的胞浆中 ;Bcl 2蛋白则分布于表皮基底层细胞的胞浆内。在增生性瘢痕中 ,Bax主要定位于表皮基底层细胞内 ,阳性细胞率明显低于正常皮肤水平 (P <0 .0 5 ) ;含有Bcl 2的阳性细胞主要为表皮细胞和部分成纤维细胞 ,阳性细胞率显著升高 (P <0 .0 5 )。结论增生性瘢痕的发生可能与Bcl 2蛋白过度表达、抑制细胞凋亡相关 ,而Bax表达降低 ,其介导的细胞凋亡受阻亦可能是增生性瘢痕形成的原因之一。Objective To observe the protein expression of Bax and Bcl 2 in hypertrophic scars and normal skins in order to explore their influences on hypertrophic scar formation.?Methods The expression intensity and distribution of Bax and Bcl 2 were detected with immunohistochemistry and pathological methods in 8 cases of hypertrophic scars and 8 cases of normal skins which were obtained from patients in our hospital.?Results In normal skin, Bax was mainly located in the cytoplasms of epidermal basal cells and some fibroblasts. Bcl 2 was mostly distributed in epidermal basal cells. In hypertrophic scars, the positive cellular ratio of Bax was significantly reduced (P<0.05). The protein particles were sited in epidermal keratinocytes. The cells which could express Bcl 2 protein were chiefly epidermal cells and some fibroblasts. The expression ratio of Bcl 2 was notably elevated in comparison with normal skin(P<0.05).?Conclusion The formation of hypertrophic scars might be associated with the increment of Bcl 2 contents and the reduction of Bax expression which lead to the inhibition of reparative cell apoptosis.

关 键 词：增生性瘢痕 BAX蛋白 BCL-2蛋白 细胞凋亡 

分 类 号：R619.6[医药卫生—外科学]